BMRB Entry 7018

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7018

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Title:
chemical shift values of the methyl groups of Met residues in the Ca2+-CaM-CaMKI-peptide complex
Deposition date:
2005-08-30
Original release date:
2013-02-15
Authors:
Yuan, Tao; Gomes, Aldrin; Barnes, Junor; Hunter, Howard; Vogel, Hans
Citation:

Citation: Yuan, Tao; Gomes, Aldrin; Barnes, Junor; Hunter, Howard; Vogel, Hans. "Spectroscopic characterization of the calmodulin-binding and autoinhibitory domains of calcium/calmodulin-dependent protein kinase I"  Arch. Biochem. Biophys. 421, 192-206 (2004).
PubMed: 14984199

Assembly members:

Assembly members:
calmodulin, polymer, 148 residues, Formula weight is not available
Cys-CaMKI peptide, polymer, 37 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mammalia   Taxonomy ID: 40674   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
Cys-CaMKI peptide: KNIHQSVESEQIKKNFAKSK WKQAFNATAVVRHMRKL

Data sets:
Data typeCount
13C chemical shifts9
1H chemical shifts27

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1.1
2.2

Entities:

Entity 1, . 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, . 37 residues - Formula weight is not available

1   LYSASNILEHISGLNSERVALGLUSERGLU
2   GLNILELYSLYSASNPHEALALYSSERLYS
3   TRPLYSGLNALAPHEASNALATHRALAVAL
4   VALARGHISMETARGLYSLEU

Samples:

sample_1: calmodulin; Cys-CaMKI peptide

conditions_1: pH*: 5.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
unknownsample_1isotropicconditions_1
unknownsample_1isotropicconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 500 MHz

Related Database Links:

BMRB 15183 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 26503 4056 5227 547 6541 6778 6798 6802 6825 6830 6831 7028 7029 7030 7031 7416 7417 7418 7423 7424 7425 17360 5286 5287 6798
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 BAE22345 BAE41623 BAG37428 BAG70091 BAG70221
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 AAA66944 AAA99458 AAH14825 AAH71177 AAI06755
PIR JC1305 MCON
PRF 0409298A 0608335A 2024225A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 NP_001070336 NP_001177124 NP_001244800 NP_003647 NP_598687
SP O02367 O16305 O96081 P02594 P05932 Q14012 Q63450 Q91YS8
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988 DAA17160
AlphaFold O02367 O16305 O96081 P02594 P05932 Q14012 Q63450 Q91YS8