BMRB Entry 7028
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7028
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Citation: Findlay, Wendy; Gradwell, Michael; Bayley, Peter. "Role of the N-terminal region of the skeletal muscle myosin light chain kinase
target sequence in its interaction with calmodulin" Protein Sci. 4, 2375-2382 (1995).
PubMed: 8563635
Assembly members:
calmodulin, polymer, 148 residues, Formula weight is not available
WFF peptide, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: Fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFYEAFSLFD
KDGDGTITTKELGTVMRSLG
QNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTD
SEEEIREAFRVFDKDGNGFI
SAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEE
FVQMMTAK
WFF peptide: KRRWKKNFIAVSAANRFK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 8 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CaM | 1 |
| 2 | WFF | 2 |
Entities:
Entity 1, CaM 148 residues - Formula weight is not available
| 1 | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ALA | ||||
| 2 | GLU | PHE | TYR | GLU | ALA | PHE | SER | LEU | PHE | ASP | ||||
| 3 | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | LYS | ||||
| 4 | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | GLY | ||||
| 5 | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ASP | ||||
| 6 | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ASN | ||||
| 7 | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | THR | ||||
| 8 | MET | MET | ALA | ARG | LYS | MET | LYS | ASP | THR | ASP | ||||
| 9 | SER | GLU | GLU | GLU | ILE | ARG | GLU | ALA | PHE | ARG | ||||
| 10 | VAL | PHE | ASP | LYS | ASP | GLY | ASN | GLY | PHE | ILE | ||||
| 11 | SER | ALA | ALA | GLU | LEU | ARG | HIS | VAL | MET | THR | ||||
| 12 | ASN | LEU | GLY | GLU | LYS | LEU | THR | ASP | GLU | GLU | ||||
| 13 | VAL | ASP | GLU | MET | ILE | ARG | GLU | ALA | ASP | ILE | ||||
| 14 | ASP | GLY | ASP | GLY | GLN | VAL | ASN | TYR | GLU | GLU | ||||
| 15 | PHE | VAL | GLN | MET | MET | THR | ALA | LYS |
Entity 2, WFF 18 residues - Formula weight is not available
| 1 | LYS | ARG | ARG | TRP | LYS | LYS | ASN | PHE | ILE | ALA | ||||
| 2 | VAL | SER | ALA | ALA | ASN | ARG | PHE | LYS |
Samples:
sample_1: calmodulin 1 mM; Tris 10 mM; KCl 100 mM; CaCl2 10 mM; WFF peptide 1 mM
conditions_1: pH*: 7.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| unknown | sample_1 | isotropic | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian UnityPlus 500 MHz
Related Database Links:
| BMRB | 15183 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 26503 4056 5227 547 6541 6778 6798 6802 6825 6830 6831 7018 7029 7030 7031 7416 7417 7418 7423 7424 7425 18556 |
| PDB | |
| DBJ | BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 BAD30083 BAD30084 BAD30085 BAD30086 BAI47180 |
| EMBL | CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 CAA46981 CAC81354 |
| GB | AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 AAA31400 AAC71016 AAC83671 AAH07753 AAH19408 |
| PIR | JC1305 MCON |
| REF | NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 NP_001074513 NP_001075705 NP_001077188 NP_149109 XP_001499833 |
| SP | O02367 O16305 O96081 P02594 P05932 A4IFM7 P07313 P20689 Q8VCR8 Q9H1R3 |
| TPG | DAA13808 DAA18029 DAA19590 DAA24777 DAA24988 DAA23271 |
| PRF | 1507147A |
| AlphaFold | O02367 O16305 O96081 P02594 P05932 A4IFM7 P07313 P20689 Q8VCR8 Q9H1R3 |