BMRB Entry 7030
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7030
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Citation: Findlay, Wendy; Gradwell, Michael; Bayley, Peter. "Role of the N-terminal region of the skeletal muscle myosin light chain kinase
target sequence in its interaction with calmodulin" Protein Sci. 4, 2375-2382 (1995).
PubMed: 8563635
Assembly members:
calmodulin, polymer, 148 residues, Formula weight is not available
FFW peptide, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: Fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFYEAFSLFD
KDGDGTITTKELGTVMRSLG
QNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTD
SEEEIREAFRVFDKDGNGFI
SAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEE
FVQMMTAK
FFW peptide: KRRFKKNFIAVSAANRWK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 8 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CaM | 1 |
| 2 | FFW | 2 |
Entities:
Entity 1, CaM 148 residues - Formula weight is not available
| 1 | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ALA | ||||
| 2 | GLU | PHE | TYR | GLU | ALA | PHE | SER | LEU | PHE | ASP | ||||
| 3 | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | LYS | ||||
| 4 | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | GLY | ||||
| 5 | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ASP | ||||
| 6 | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ASN | ||||
| 7 | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | THR | ||||
| 8 | MET | MET | ALA | ARG | LYS | MET | LYS | ASP | THR | ASP | ||||
| 9 | SER | GLU | GLU | GLU | ILE | ARG | GLU | ALA | PHE | ARG | ||||
| 10 | VAL | PHE | ASP | LYS | ASP | GLY | ASN | GLY | PHE | ILE | ||||
| 11 | SER | ALA | ALA | GLU | LEU | ARG | HIS | VAL | MET | THR | ||||
| 12 | ASN | LEU | GLY | GLU | LYS | LEU | THR | ASP | GLU | GLU | ||||
| 13 | VAL | ASP | GLU | MET | ILE | ARG | GLU | ALA | ASP | ILE | ||||
| 14 | ASP | GLY | ASP | GLY | GLN | VAL | ASN | TYR | GLU | GLU | ||||
| 15 | PHE | VAL | GLN | MET | MET | THR | ALA | LYS |
Entity 2, FFW 18 residues - Formula weight is not available
| 1 | LYS | ARG | ARG | PHE | LYS | LYS | ASN | PHE | ILE | ALA | ||||
| 2 | VAL | SER | ALA | ALA | ASN | ARG | TRP | LYS |
Samples:
sample_1: calmodulin 1 mM; Tris 10 mM; KCl 100 mM; CaCl2 10 mM; FFW peptide 1 mM
conditions_1: pH*: 7.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| unknown | sample_1 | isotropic | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian UnityPlus 500 MHz
Related Database Links:
| BMRB | 15183 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 26503 4056 5227 547 6541 6778 6798 6802 6825 6830 6831 7018 7028 7029 7031 7416 7417 7418 7423 7424 7425 |
| PDB | |
| DBJ | BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 |
| EMBL | CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 |
| GB | AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 |
| PIR | JC1305 MCON |
| REF | NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 |
| SP | O02367 O16305 O96081 P02594 P05932 |
| TPG | DAA13808 DAA18029 DAA19590 DAA24777 DAA24988 |
| AlphaFold | O02367 O16305 O96081 P02594 P05932 |