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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17771
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Irene, Deli; Huang, Jian-Wen; Chung, Tse-Yu; Li, Feng-Yin; Tzen, Jason T-C; Lin, Ta-Hsien; Chyan, Chia-Lin. "Binding orientation and specificity of calmodulin to rat olfactory cyclic nucleotide-gated ion channel." J. Biomol. Struct. Dyn. 31, 414-425 (2013).
PubMed: 22877078
Assembly members:
Calmodulin, polymer, 148 residues, Formula weight is not available
OLFp, polymer, 28 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29c
Data type | Count |
13C chemical shifts | 1371 |
15N chemical shifts | 348 |
1H chemical shifts | 2278 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Chain 1 | 1 |
2 | Chain 2 | 2 |
3 | Calcium ion | 3 |
Entity 1, Chain 1 148 residues - Formula weight is not available
1 | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ALA | ||||
2 | GLU | PHE | LYS | GLU | ALA | PHE | SER | LEU | PHE | ASP | ||||
3 | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | LYS | ||||
4 | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | GLY | ||||
5 | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ASP | ||||
6 | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ASN | ||||
7 | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | THR | ||||
8 | MET | MET | ALA | ARG | LYS | MET | LYS | ASP | THR | ASP | ||||
9 | SER | GLU | GLU | GLU | ILE | ARG | GLU | ALA | PHE | ARG | ||||
10 | VAL | PHE | ASP | LYS | ASP | GLY | ASN | GLY | TYR | ILE | ||||
11 | SER | ALA | ALA | GLU | LEU | ARG | HIS | VAL | MET | THR | ||||
12 | ASN | LEU | GLY | GLU | LYS | LEU | THR | ASP | GLU | GLU | ||||
13 | VAL | ASP | GLU | MET | ILE | ARG | GLU | ALA | ASP | ILE | ||||
14 | ASP | GLY | ASP | GLY | GLN | VAL | ASN | TYR | GLU | GLU | ||||
15 | PHE | VAL | GLN | MET | MET | THR | ALA | LYS |
Entity 2, Chain 2 28 residues - Formula weight is not available
1 | THR | PRO | ARG | ARG | GLY | ARG | GLY | GLY | PHE | GLN | ||||
2 | ARG | ILE | VAL | ARG | LEU | VAL | GLY | VAL | ILE | ARG | ||||
3 | ASP | TRP | ALA | ASN | LYS | ASN | PHE | ARG |
Entity 3, Calcium ion - Ca - 40.078 Da.
1 | CA |
CaM-OLFp: Calmodulin, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; OLFp, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; H2O 90%; D2O 10%
sample_conditions: pH: 6.7; temperature: 310 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | CaM-OLFp | isotropic | sample_conditions |
2D 1H-13C HSQC | CaM-OLFp | isotropic | sample_conditions |
2D 1H-1H TOCSY | CaM-OLFp | isotropic | sample_conditions |
2D DQF-COSY | CaM-OLFp | isotropic | sample_conditions |
2D 1H-1H NOESY | CaM-OLFp | isotropic | sample_conditions |
3D HNCA | CaM-OLFp | isotropic | sample_conditions |
3D HN(CO)CA | CaM-OLFp | isotropic | sample_conditions |
3D HNCO | CaM-OLFp | isotropic | sample_conditions |
3D HN(CA)CO | CaM-OLFp | isotropic | sample_conditions |
3D HNCACB | CaM-OLFp | isotropic | sample_conditions |
3D C(CO)NH | CaM-OLFp | isotropic | sample_conditions |
3D HBHA(CO)NH | CaM-OLFp | isotropic | sample_conditions |
3D H(CCO)NH | CaM-OLFp | isotropic | sample_conditions |
3D HCCH-TOCSY | CaM-OLFp | isotropic | sample_conditions |
3D HCCH-COSY | CaM-OLFp | isotropic | sample_conditions |
3D 1H-15N-edited TOCSY HSQC | CaM-OLFp | isotropic | sample_conditions |
3D simultaneous 1H-13C/15N-edited NOESY-HSQC | CaM-OLFp | isotropic | sample_conditions |
AURELIA, Neidig, Geyer, Gorler, Antz, Saffrich, Beneicke, Kalbitzer - chemical shift assignment
BMRB | 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4270 4284 4310 |
PDB | |
DBJ | BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 |
EMBL | CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 CAA39135 CAQ34767 |
GB | AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 AAD41473 |
PIR | JC1305 MCON |
PRF | 0409298A 0608335A 1614345A |
REF | NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 NP_037060 XP_008756353 |
SP | O02367 O16305 O96081 P02594 P05932 Q00195 |
TPG | DAA13808 DAA18029 DAA19590 DAA24777 DAA24988 |
AlphaFold | O02367 O02367 P05932 P05932 P02594 P02594 O96081 O96081 O16305 O16305 Q00195 Q00195 |
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