BMRB Entry 16418

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16418

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Title:
Interaction between calcium-free calmodulin and IQ motif of neurogranin studied by nuclear magnetic resonance spectroscopy
Deposition date:
2009-07-23
Original release date:
2010-03-08
Authors:
Cui, Yanfang; Wen, Jian; Sze, Kong Hung; Man, David; Lin, Donghai; Liu, Maili; Zhu, Guang
Citation:

Citation: Cui, Yanfang; Wen, Jian; Sze, Kong Hung; Man, David; Lin, Donghai; Liu, Maili; Zhu, Guang. "Interaction between calcium-free calmodulin and IQ motif of neurogranin studied by nuclear magnetic resonance spectroscopy"  Anal. Biochem. 315, 175-182 (2003).
PubMed: 12689827

Assembly members:

Assembly members:
apoCaM, polymer, 148 residues, Formula weight is not available
IQ_peptide, polymer, 24 residues, 2860 Da.

Natural source:

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: JM105

Entity Sequences (FASTA):

Entity Sequences (FASTA):
apoCaM: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
IQ_peptide: ANAAAAKIQASFRGHMARKK IKSG

Data sets:
  • binding_constants
Data typeCount
binding constants15

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apoCaM1
2IQ_peptide2

Entities:

Entity 1, apoCaM 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, IQ_peptide 24 residues - 2860 Da.

1   ALAASNALAALAALAALALYSILEGLNALA
2   SERPHEARGGLYHISMETALAARGLYSLYS
3   ILELYSSERGLY

Samples:

sample_1: apoCaM, [U-13C; U-15N], 1 mM; K2HPO4 20 mM; dithiothreitol 4 mM; EDTA 5 mM; D2O 10%; H2O 90%

sample_2: apoCaM, [U-13C; U-15N], 0.99 mM; K2HPO4 20 mM; dithiothreitol 4 mM; EDTA 5 mM; D2O 10%; H2O 90%; IQ_peptide 0.28 mM

sample_3: apoCaM, [U-13C; U-15N], 0.98 mM; K2HPO4 20 mM; dithiothreitol 4 mM; EDTA 5 mM; D2O 10%; H2O 90%; IQ_peptide 0.83 mM

sample_4: apoCaM, [U-13C; U-15N], 0.97 mM; K2HPO4 20 mM; dithiothreitol 4 mM; EDTA 5 mM; D2O 10%; H2O 90%; IQ_peptide 1.09 mM

sample_5: apoCaM, [U-13C; U-15N], 0.94 mM; K2HPO4 20 mM; dithiothreitol 4 mM; EDTA 5 mM; D2O 10%; H2O 90%; IQ_peptide 2.12 mM

sample_6: apoCaM, [U-13C; U-15N], 0.93 mM; K2HPO4 20 mM; dithiothreitol 4 mM; EDTA 5 mM; D2O 10%; H2O 90%; IQ_peptide 2.61 mM

sample_7: apoCaM, [U-13C; U-15N], 0.92 mM; K2HPO4 20 mM; dithiothreitol 4 mM; EDTA 5 mM; D2O 10%; H2O 90%; IQ_peptide 2.85 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_5isotropicsample_conditions_1
2D 1H-15N HSQCsample_6isotropicsample_conditions_1
2D 1H-15N HSQCsample_7isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian Unity INOVA 750 MHz

Related Database Links:

BMRB 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4270 4284 4310
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 BAB22466 BAE34589
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 CAA67533 CAA70867 CAA75343 CAC37631
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 AAA42023 AAA80223 AAB28130 AAB34481 AAB49458
PIR JC1305 MCON
PRF 0409298A 0608335A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 NP_001106784 NP_001119653 NP_001254528 NP_006167 NP_071312
SP O02367 O16305 O96081 P02594 P05932 P35722 P54877 P60761 Q04940 Q92686
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988 DAA13911
AlphaFold O02367 O16305 O96081 P02594 P05932 P35722 P54877 P60761 Q04940 Q92686