BMRB Entry 5286

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5286

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Title:
Backbone 1H, 13C, and 15N Chemical shift assigments for [13C,15N]CaM:CaMKIp complex
Deposition date:
2002-02-12
Original release date:
2002-06-13
Authors:
Kranz, James; Lee, Eun; Nairn, Angus; Wand, A.
Citation:

Citation: Kranz, James; Lee, Eun; Nairn, Angus; Wand, A.. "A Direct Test of the Reductionist Approach to Structural Studies of Calmodulin Activity: Relevance of Peptide Models of Target Proteins"  J. Biol. Chem. 277, 16351-16354 (2002).
PubMed: 11904288

Assembly members:

Assembly members:
calmodulin, polymer, 148 residues, 16706 Da.
CaM-binding sequence(299-320) from CaMKI, polymer, 23 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
CaM-binding sequence(299-320) from CaMKI: XKSKWKQAFNATAVVRHMRK LQX

Data sets:
Data typeCount
1H chemical shifts144
13C chemical shifts430
15N chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1
2CaM-dependent kinase I (299-320)2

Entities:

Entity 1, calmodulin 148 residues - 16706 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, CaM-dependent kinase I (299-320) 23 residues - Formula weight is not available

1   AYALYSSERLYSTRPLYSGLNALAPHEASN
2   ALATHRALAVALVALARGHISMETARGLYS
3   LEUGLNNH2

Samples:

sample_1: calmodulin, [U-99% 13C; U-99% 15N], 2 mM; CaM-binding sequence(299-320) from CaMKI 2.25 mM

Ex-cond_1: pH: 6.5; temperature: 303 K; ionic strength: 0.16 M

Experiments:

NameSampleSample stateSample conditions
1H-15N-HSQCsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HN(CA)COsample_1not availableEx-cond_1

Software:

felix v97.0 -

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

BMRB 17360 5287 6798 7018 5287
PDB
DBJ BAE22345 BAE41623 BAG37428 BAG70091 BAG70221 BAE41623 BAG37428 BAE22345
GB AAA66944 AAA99458 AAH14825 AAH71177 AAI06755
PRF 2024225A 2024225A
REF NP_001070336 NP_001177124 NP_001244800 NP_003647 NP_598687 XP_001095449 NP_598687 NP_604463 NP_001070336 NP_003647
SP Q14012 Q63450 Q91YS8
TPG DAA17160
SWISS-PROT Q63450 Q91YS8 Q14012
GenBank AAI06755 AAH14825 AAH71177 AAA66944 AAA99458
AlphaFold Q14012 Q63450 Q91YS8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks