BMRB Entry 547

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR547

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Title:
A Novel Approach for Sequential Assignment of 1H, 13C, and 15N Spectra of Larger Proteins: Heteronuclear Triple-Resonance Three-Dimensional NMR Spectroscopy. Application to Calmodulin
Deposition date:
1995-07-31
Original release date:
1995-07-31
Authors:
Ikura, Mitsuhiko; Kay, Lewis; Bax, Ad
Citation:

Citation: Ikura, Mitsuhiko; Kay, Lewis; Bax, Ad. "A Novel Approach for Sequential Assignment of 1H, 13C, and 15N Spectra of Larger Proteins: Heteronuclear Triple-Resonance Three-Dimensional NMR Spectroscopy. Application to Calmodulin"  Biochemistry 29, 4659-4667 (1990).

Assembly members:

Assembly members:
calmodulin, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruitfly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: not available   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGFI SAAELRHVMTNLGEKLTDEE VDEMIREANIDGDGQVNYEE FVTMMTSK

Data sets:
Data typeCount
13C chemical shifts296
15N chemical shifts147
1H chemical shifts303

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1

Entities:

Entity 1, calmodulin 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYPHEILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASNILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALTHRMETMETTHRSERLYS

Samples:

sample_one:

sample_condition_set_one: pH: 6.3 na; temperature: 320 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 15184 15185 15186 15191 15470 15624 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 26503 4056 5227 6541 6778 6798 6802 6825 6830 6831 7018 7028 7029 7030 7031
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863
PIR JC1305 MCON
PRF 0711223A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234
SP O02367 O16305 O96081 P02595 P05932
TPG DAA19590 DAA24777 DAA24988
AlphaFold O02367 O16305 O96081 P02595 P05932

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks