BMRB Entry 4056

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4056

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Title:
Solution Structure of Calmodulin-W-7 Complex: The Basis of Diversity in Molecular Recognition
Deposition date:
1997-09-04
Original release date:
1998-09-03
Authors:
Osawa, Masanori; Swindells, Mark; Tanikawa, Jun; Tanaka, Toshiyuki; Toshiyuki, Tanaka; Mase, Toshiyasu; Furuya, Toshio; Ikura, Mitsuhiko
Citation:

Citation: Osawa, Masanori; Swindells, Mark; Tanikawa, Jun; Toshiyuki, Tanaka; Toshiyasu, Mase; Toshio, Furuya; Mitsuhiko, Ikura. "Solution Structure of Calmodulin-W-7 Complex: The Basis of Diversity in Molecular Recognition"  J. Mol. Biol. ., .-. (1998).

Assembly members:

Assembly members:
calmodulin, polymer, 148 residues, 18000 Da.
WW7, non-polymer, 340.868 Da.

Natural source:

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK

Data typeCount
13C chemical shifts560
15N chemical shifts135
1H chemical shifts911

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1
2W-72

Entities:

Entity 1, calmodulin 148 residues - 18000 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, W-7 - C16 H21 Cl N2 O2 S - 340.868 Da.

1   WW7

Samples:

sample_one: calmodulin, [U-95%-13C], 1.5 mM; W-7 7.5 mM; potassium chloride 100 mM; calcium chloride 10.6 mM

sample_two: calmodulin, [U-95%-15N], 1.5 mM; W-7 7.5 mM; potassium chloride 100 mM; calcium chloride 10.6 mM

sample_conditions_set_1: pH: 6.8; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions_set_1

Software:

No software information available

NMR spectrometers:

    Related Database Links:

    BMRB 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4270 4284 4310
    PDB
    DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788
    EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120
    GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863
    PIR JC1305 MCON
    PRF 0409298A 0608335A
    REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234
    SP O02367 O16305 O96081 P02594 P05932
    TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988
    AlphaFold O02367 O16305 O96081 P02594 P05932

    Download HSQC peak lists in one of the following formats:
    CSV: Backbone or all simulated peaks
    SPARKY: Backbone or all simulated peaks