BMRB Entry 6541

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6541

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Title:
1H, 13C and 15N chemical shift assignments for stereo array isotope labeled (SAIL) calmodulin
Deposition date:
2005-03-09
Original release date:
2006-04-25
Authors:
Kainosho, Masatsune; Torizawa, Takuya; Terauchi, Tsutomu; Ono, Akira Mei; Guntert, Peter
Citation:

Citation: Kainosho, Masatsune; Torizawa, Takuya; Iwashita, Yuki; Terauchi, Tsutomu; Ono, Akira Mei; Guntert, Peter. "Optimal isotope labelling for NMR protein structure determinations"  Nature 440, 52-57 (2006).
PubMed: 16511487

Assembly members:

Assembly members:
calmodulin, polymer, 148 residues, 40.078 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:

Experimental source:   Production method: chemical, enzymatic, and cell-free synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK

Data sets:
Data typeCount
13C chemical shifts591
15N chemical shifts158
1H chemical shifts751

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1
2Ca ion, 12
3Ca ion, 22
4Ca ion, 32
5Ca ion, 42

Entities:

Entity 1, calmodulin 148 residues - 40.078 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, Ca ion, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: calmodulin, SAIL (stereo array isotope labeled), 0.7 mM; MES-d13 5 mM; bis-Tris-d19 10 mM; CaCl2 5 mM; NaN3 0.1 mM

conditions_1: pH: 6.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
[1H,15N]-HSQCsample_1not availableconditions_1
[1H,13C]-HSQCsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
CBCANHsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HBHANHsample_1not availableconditions_1
HBHA(CO)NHsample_1not availableconditions_1
CBCGHEsample_1not availableconditions_1
HBCBCGHEsample_1not availableconditions_1
15N-resolved [1H,1H]-NOESYsample_1not availableconditions_1
13C-resolved [1H,1H]-NOESYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 15183 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 26503 4056 5227 547 6778 6798 6802 6825 6830 6831 7018 7028 7029 7030 7031 7416 7417 7418 7423 7424 7425
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863
PIR JC1305 MCON
PRF 0409298A 0608335A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234
SP O02367 O16305 O96081 P02594 P05932
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988
AlphaFold O02367 O16305 O96081 P02594 P05932

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks