BMRB Entry 6831

Title:
1H, 13C Chemical shifts and pKa values in Ca2+-CaM and the CaM-cNOS peptide comlex.
Deposition date:
2005-09-21
Original release date:
2013-02-15
Authors:
Zhang, Mingjie; Yuan, Tao; Aramini, James; Vogel, Hans
Citation:

Citation: Zhang, Mingjie; Yuan, Tao; Aramini, James; Vogel, Hans. "Interaction of Calmodulin with Its Binding Domain of Rat Cerebellar Nitric Oxide Synthase"  J. Biol. Chem. 270, 20901-20907 (1995).
PubMed: 7545663

Assembly members:

Assembly members:
calmodulin, polymer, 148 residues, Formula weight is not available
cNOS peptide, polymer, 23 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts9
1H chemical shifts27

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1
2cNOS peptide2

Entities:

Entity 1, calmodulin 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, cNOS peptide 23 residues - Formula weight is not available

1   LYSARGARGALAILEGLYPHELYSLYSLEU
2   ALAGLUALAVALLYSPHESERALALYSLEU
3   METGLYGLN

Samples:

sample_1: calmodulin, [U-13CE]-Met, ; D2O 99.9%; KCl 100 mM; cNOS peptide

conditions_1: pD: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
unknownsample_1isotropicconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

BMRB 15183 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 26503 4056 5227 547 6541 6778 6798 6802 6825 6830 7018 7028 7029 7030 7031 7416 7417 7418 7423 7424 7425 15191
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 BAA03415 BAA03895 BAG57679
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 CAA42574
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 AAA36376 AAA62405 AAB49040 AAB60654 AAB68663
PIR JC1305 MCON
PRF 0409298A 0608335A 1712320A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 NP_000611 NP_001075854 NP_001182074 NP_001191142 NP_001191143
SP O02367 O16305 O96081 P02594 P05932 O19132 P29475 P29476 Q9Z0J4
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988 DAA20749
AlphaFold P02594 O02367 O16305 O96081 P05932 Q9Z0J4 P29476 P29475 O19132