BMRB Entry 15470
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15470
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Citation: Rodriguez-Castaneda, Fernando; Coudevylle, Nicolas; Becker, Stefan; Brose, Nils; Carlomagno, Teresa; Griesinger, Christian. "(1)H, (13)C and (15)N resonance assignments of the Calmodulin-Munc13-1 peptide complex." Biomol. NMR assignments 4, 45-48 (2009).
PubMed: 20013162
Assembly members:
calmodulin, polymer, 148 residues, 16700 Da.
Munc13-1_(458-492), polymer, 36 residues, 4170 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: African clawed frog Taxonomy ID: 8355 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Xenopus laevis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a and pGEX-2T
Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFKEAFSLFD
KDGDGTITTKELGTVMRSLG
QNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTD
SEEEIREAFRVFDKDGNGYI
SAAELRHVMTNLGEKLTDEE
VDEMIREANIDGDGQVNYEE
FVQMMTAK
Munc13-1_(458-492): GSRAKANWLRAFNKVRMQLQ
EARGEGEMSKSLWFKG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 666 |
| 15N chemical shifts | 191 |
| 1H chemical shifts | 1105 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | calmodulin | 1 |
| 2 | Munc13-1 | 2 |
| 3 | Calcium I | 3 |
| 4 | Calcium II | 3 |
| 5 | Calcium III | 3 |
| 6 | Calcium IV | 3 |
Entities:
Entity 1, calmodulin 148 residues - 16700 Da.
| 1 | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ALA | ||||
| 2 | GLU | PHE | LYS | GLU | ALA | PHE | SER | LEU | PHE | ASP | ||||
| 3 | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | LYS | ||||
| 4 | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | GLY | ||||
| 5 | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ASP | ||||
| 6 | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ASN | ||||
| 7 | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | THR | ||||
| 8 | MET | MET | ALA | ARG | LYS | MET | LYS | ASP | THR | ASP | ||||
| 9 | SER | GLU | GLU | GLU | ILE | ARG | GLU | ALA | PHE | ARG | ||||
| 10 | VAL | PHE | ASP | LYS | ASP | GLY | ASN | GLY | TYR | ILE | ||||
| 11 | SER | ALA | ALA | GLU | LEU | ARG | HIS | VAL | MET | THR | ||||
| 12 | ASN | LEU | GLY | GLU | LYS | LEU | THR | ASP | GLU | GLU | ||||
| 13 | VAL | ASP | GLU | MET | ILE | ARG | GLU | ALA | ASN | ILE | ||||
| 14 | ASP | GLY | ASP | GLY | GLN | VAL | ASN | TYR | GLU | GLU | ||||
| 15 | PHE | VAL | GLN | MET | MET | THR | ALA | LYS |
Entity 2, Munc13-1 36 residues - 4170 Da.
| 1 | GLY | SER | ARG | ALA | LYS | ALA | ASN | TRP | LEU | ARG | ||||
| 2 | ALA | PHE | ASN | LYS | VAL | ARG | MET | GLN | LEU | GLN | ||||
| 3 | GLU | ALA | ARG | GLY | GLU | GLY | GLU | MET | SER | LYS | ||||
| 4 | SER | LEU | TRP | PHE | LYS | GLY |
Entity 3, Calcium I - Ca - 40.078 Da.
| 1 | CA |
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks