BMRB Entry 25253

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25253

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Title:
Chemical Shifts of Y99E,N111D mutant CaM with iNOS
Deposition date:
2014-09-29
Original release date:
2015-09-04
Authors:
Piazza, Michael; Dieckmann, Thorsten
Citation:

Citation: Piazza, Michael; Dieckmann, Thorsten; Guillemette, Guy. "Chemical shift perturbations induced by residue specific mutations of CaM interacting with NOS peptides"  Biomol. NMR Assign. 9, 299-302 (2015).
PubMed: 25604396

Assembly members:

Assembly members:
CaM, polymer, 148 residues, Formula weight is not available
iNOS, polymer, 17 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CaM: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGEI SAAELRHVMTDLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
iNOS: LKVLVKAVLFACMLMRK

Data sets:
Data typeCount
15N chemical shifts144
1H chemical shifts772

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CaM1
2iNOS2

Entities:

Entity 1, CaM 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYGLUILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASPLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, iNOS 17 residues - Formula weight is not available

1   LEULYSVALLEUVALLYSALAVALLEUPHE
2   ALACYSMETLEUMETARGLYS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks