BMRB Entry 26627

Name: Backbone 1H, 13C and 15N resonance assignments for CaM-Fas-47-83 complex
Published: 2015-09-04

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26627

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N resonance assignments for CaM-Fas-47-83 complex

Deposition date:

2015-08-07

Original release date:

2015-09-04

Authors:

Vlach, Jiri; Saad, Jamil; Chang, Bliss; Samal, Alexandra; Fernandez, Timothy; Brooke, Dewey; Prevelige, Peter

Citation:

Citation: Chang, Bliss; Samal, Alexandra; Vlach, Jiri; Fernandez, Timothy; Brooke, Dewey; Prevelige, Peter; Saad, Jamil. "Identification of the Calmodulin-Binding Domains of Fas Death Receptor" PLoS One 11, e0146493-e0146493 (2016).
PubMed: 26735300

Assembly members:

Assembly members:
CaM, polymer, 148 residues, 16683.1669 Da.
Fas47-83, polymer, 39 residues, 4568.261 Da.

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pT7-7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CaM: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
Fas47-83: SVQDTAEQKVQLLRNWHQLH GKKEAYDTLIKDLKKANLC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	376
15N chemical shifts	179
1H chemical shifts	283

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CaM	1
2	Fas47-83	2

Entities:

Entity 1, CaM 148 residues - 16683.1669 Da.

1

ALA

ASP

GLN

LEU

THR

GLU

GLN

ILE

ALA

2

GLU

PHE

LYS

GLU

ALA

PHE

SER

LEU

PHE

ASP

3

LYS

ASP

GLY

ASP

GLY

THR

ILE

THR

LYS

4

GLU

LEU

GLY

THR

VAL

MET

ARG

SER

LEU

GLY

5

GLN

ASN

PRO

THR

GLU

ALA

GLU

LEU

GLN

ASP

6

MET

ILE

ASN

GLU

VAL

ASP

ALA

ASP

GLY

ASN

7

GLY

THR

ILE

ASP

PHE

PRO

GLU

PHE

LEU

THR

8

MET

ALA

ARG

LYS

MET

LYS

ASP

THR

ASP

9

SER

GLU

ILE

ARG

GLU

ALA

PHE

ARG

10

VAL

PHE

ASP

LYS

ASP

GLY

ASN

GLY

TYR

ILE

11

SER

ALA

GLU

LEU

ARG

HIS

VAL

MET

THR

12

ASN

LEU

GLY

GLU

LYS

LEU

THR

ASP

GLU

13

VAL

ASP

GLU

MET

ILE

ARG

GLU

ALA

ASP

ILE

14

ASP

GLY

ASP

GLY

GLN

VAL

ASN

TYR

GLU

15

PHE

VAL

GLN

MET

THR

ALA

LYS

Entity 2, Fas47-83 39 residues - 4568.261 Da.

Serine 1 is an artefact from SUMO-Fas fusion protein. Peptide is a fragment of FAS death domain, FAS residues 267-304.

1

SER

VAL

GLN

ASP

THR

ALA

GLU

GLN

LYS

VAL

2

GLN

LEU

ARG

ASN

TRP

HIS

GLN

LEU

HIS

3

GLY

LYS

GLU

ALA

TYR

ASP

THR

LEU

ILE

4

LYS

ASP

LEU

LYS

ALA

ASN

LEU

CYS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	15N	isotropic	cond
3D 1H-15N NOESY	15N	isotropic	cond
3D HNCA	13C15N	isotropic	cond
3D HNCACB	13C15N	isotropic	cond
cf_HNcoCA (H[N[co[{CA\|ca[C]}]]])	13C15N	isotropic	cond
cf_HNcoCACB (H[N[co[{CA\|ca[C]}]]])	13C15N	isotropic	cond
3D 1H-15N TOCSY	13C15N_2	isotropic	cond
3D HNCO	13C15N_2	isotropic	cond

GenBank	TmpAcc
NCBI	NP_000034.1

BMRB Entry 26627

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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