BMRB Entry 26627

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26627

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Title:
Backbone 1H, 13C and 15N resonance assignments for CaM-Fas-47-83 complex
Deposition date:
2015-08-07
Original release date:
2015-09-04
Authors:
Vlach, Jiri; Saad, Jamil; Chang, Bliss; Samal, Alexandra; Fernandez, Timothy; Brooke, Dewey; Prevelige, Peter
Citation:

Citation: Chang, Bliss; Samal, Alexandra; Vlach, Jiri; Fernandez, Timothy; Brooke, Dewey; Prevelige, Peter; Saad, Jamil. "Identification of the Calmodulin-Binding Domains of Fas Death Receptor"  PLoS One 11, e0146493-e0146493 (2016).
PubMed: 26735300

Assembly members:

Assembly members:
CaM, polymer, 148 residues, 16683.1669 Da.
Fas47-83, polymer, 39 residues, 4568.261 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pT7-7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CaM: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
Fas47-83: SVQDTAEQKVQLLRNWHQLH GKKEAYDTLIKDLKKANLC

Data sets:
Data typeCount
13C chemical shifts376
15N chemical shifts179
1H chemical shifts283

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CaM1
2Fas47-832

Entities:

Entity 1, CaM 148 residues - 16683.1669 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, Fas47-83 39 residues - 4568.261 Da.

Serine 1 is an artefact from SUMO-Fas fusion protein. Peptide is a fragment of FAS death domain, FAS residues 267-304.

1   SERVALGLNASPTHRALAGLUGLNLYSVAL
2   GLNLEULEUARGASNTRPHISGLNLEUHIS
3   GLYLYSLYSGLUALATYRASPTHRLEUILE
4   LYSASPLEULYSLYSALAASNLEUCYS

Samples:

15N: CaM, [[U-95% 13C; U-95% 15N]], 0.5 mM; Fas47-83 0.5 mM; H2O 95%; D2O 5%; Tris 50 mM; NaCl 100 mM; CaCl2 5 mM

13C15N: CaM, [[U-95% 13C; U-95% 15N]], 0.5 mM; Fas47-83 0.5 mM; H2O 95%; D2O 5%; Tris 50 mM; NaCl 100 mM; CaCl2 5 mM

13C15N_2: CaM 0.3 mM; Fas47-83, [[U-95% 13C; U-95% 15N]], 0.3 mM; H2O 95%; D2O 5%; Tris 50 mM; NaCl 100 mM; CaCl2 5 mM

cond: ionic strength: 0.115 M; pH: 7.000; pressure: 1.000 atm; temperature: 308.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQC15Nisotropiccond
3D 1H-15N NOESY15Nisotropiccond
3D HNCA13C15Nisotropiccond
3D HNCACB13C15Nisotropiccond
cf_HNcoCA (H[N[co[{CA|ca[C]}]]])13C15Nisotropiccond
cf_HNcoCACB (H[N[co[{CA|ca[C]}]]])13C15Nisotropiccond
3D 1H-15N TOCSY13C15N_2isotropiccond
3D HNCO13C15N_2isotropiccond

Software:

CcpNmr_Analysis v2.4, CCPN - data analysis

nmrDraw vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum analysis, Spectrum display

nmrPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing

nmrView vany, Johnson, One Moon Scientific - Spectrum analysis, Spectrum display

NMR spectrometers:

  • Bruker DMX 700 MHz

Related Database Links:

GenBank TmpAcc
NCBI NP_000034.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks