BMRB Entry 25257

Title:
Chemical Shifts of Y99E mutant CaM with eNOS   PubMed: 25604396
Deposition date:
2014-09-30
Original release date:
2015-09-04
Authors:
Piazza, Michael; Dieckmann, Thorsten
Citation:

Citation: Piazza, Michael; Dieckmann, Thorsten; Guillemette, Guy. "Chemical shift perturbations induced by residue specific mutations of CaM interacting with NOS peptides"  Biomol. NMR Assign. 9, 299-302 (2015).

Assembly members:

Assembly members:
CaM, polymer, 148 residues, Formula weight is not available
eNOS, polymer, 17 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Experimental source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
15N chemical shifts141
1H chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CaM1
2eNOS2

Entities:

Entity 1, CaM 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYGLUILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASPLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, eNOS 17 residues - Formula weight is not available

1   LYSLYSTHRPHELYSGLUVALALAASNALA
2   VALLYSILESERALASERLEU

Samples:

sample_1: CaM, [U-99% 13C; U-99% 15N], 1 mM; eNOS 1 mM; potassium chloride 100 mM; sodium azide 0.2 mM; calcium chloride 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.150 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks