BMRB Entry 25257

Name: Chemical Shifts of Y99E mutant CaM with eNOS
Published: 2015-09-04

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25257

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shifts of Y99E mutant CaM with eNOS

Deposition date:

2014-09-30

Original release date:

2015-09-04

Authors:

Piazza, Michael; Dieckmann, Thorsten

Citation:

Citation: Piazza, Michael; Dieckmann, Thorsten; Guillemette, Guy. "Chemical shift perturbations induced by residue specific mutations of CaM interacting with NOS peptides" Biomol. NMR Assign. 9, 299-302 (2015).
PubMed: 25604396

Assembly members:

Assembly members:
CaM, polymer, 148 residues, Formula weight is not available
eNOS, polymer, 17 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CaM: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGEI SAAELRHVMTDLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
eNOS: KKTFKEVANAVKISASL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	141
1H chemical shifts	141

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CaM	1
2	eNOS	2

Entities:

Entity 1, CaM 148 residues - Formula weight is not available

1

ALA

ASP

GLN

LEU

THR

GLU

GLN

ILE

ALA

2

GLU

PHE

LYS

GLU

ALA

PHE

SER

LEU

PHE

ASP

3

LYS

ASP

GLY

ASP

GLY

THR

ILE

THR

LYS

4

GLU

LEU

GLY

THR

VAL

MET

ARG

SER

LEU

GLY

5

GLN

ASN

PRO

THR

GLU

ALA

GLU

LEU

GLN

ASP

6

MET

ILE

ASN

GLU

VAL

ASP

ALA

ASP

GLY

ASN

7

GLY

THR

ILE

ASP

PHE

PRO

GLU

PHE

LEU

THR

8

MET

ALA

ARG

LYS

MET

LYS

ASP

THR

ASP

9

SER

GLU

ILE

ARG

GLU

ALA

PHE

ARG

10

VAL

PHE

ASP

LYS

ASP

GLY

ASN

GLY

GLU

ILE

11

SER

ALA

GLU

LEU

ARG

HIS

VAL

MET

THR

12

ASP

LEU

GLY

GLU

LYS

LEU

THR

ASP

GLU

13

VAL

ASP

GLU

MET

ILE

ARG

GLU

ALA

ASP

ILE

14

ASP

GLY

ASP

GLY

GLN

VAL

ASN

TYR

GLU

15

PHE

VAL

GLN

MET

THR

ALA

LYS

Entity 2, eNOS 17 residues - Formula weight is not available

1

LYS

THR

PHE

LYS

GLU

VAL

ALA

ASN

ALA

2

VAL

LYS

ILE

SER

ALA

SER

LEU

Samples:

sample_1: CaM, [U-99% 13C; U-99% 15N], 1 mM; eNOS 1 mM; potassium chloride 100 mM; sodium azide 0.2 mM; calcium chloride 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.150 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks