BMRB Entry 4310
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4310
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Larsson, Goran; Schleucher, Jurgen; Onions, Jacquline; Hermann, Stefan; Grundstrom, Thomas; Wijmenga, Sybren. "A Novel Target Recognition Revealed by Calmodulin in Complex with the Basic
Helix-loop-helix Transcription Factor SEF2-1/E2-2" Protein Sci. 10, 169-186 (2001).
PubMed: 11266605
Assembly members:
calmodulin, polymer, 148 residues, 16700 Da.
SL3 Enhancer Factor2-1, polymer, 21 residues, 2500 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: plasmid
Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFKEAFSLFD
KDGDGTITTKELGTVMRSLG
QNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTD
SEEEIREAFRVFDKDGNGYI
SAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEE
FVQMMTAK
SL3 Enhancer Factor2-1: KERRMANNARERLRVRGGCG
W
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 577 |
| 15N chemical shifts | 144 |
| 1H chemical shifts | 1139 |
| coupling constants | 125 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | calmodulin one | 1 |
| 2 | calmodulin two | 1 |
| 3 | SL3 Enhancer Factor2-1 one | 2 |
| 4 | SL3 Enhancer Factor2-1 two | 2 |
| 5 | CALCIUM ION, I | 3 |
| 6 | CALCIUM ION, II | 3 |
| 7 | CALCIUM ION, III | 3 |
| 8 | CALCIUM ION, IV | 3 |
| 9 | CALCIUM ION, V | 3 |
| 10 | CALCIUM ION, VI | 3 |
| 11 | CALCIUM ION, VII | 3 |
| 12 | CALCIUM ION, VIII | 3 |
Entities:
Entity 1, calmodulin one 148 residues - 16700 Da.
| 1 | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ALA | ||||
| 2 | GLU | PHE | LYS | GLU | ALA | PHE | SER | LEU | PHE | ASP | ||||
| 3 | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | LYS | ||||
| 4 | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | GLY | ||||
| 5 | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ASP | ||||
| 6 | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ASN | ||||
| 7 | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | THR | ||||
| 8 | MET | MET | ALA | ARG | LYS | MET | LYS | ASP | THR | ASP | ||||
| 9 | SER | GLU | GLU | GLU | ILE | ARG | GLU | ALA | PHE | ARG | ||||
| 10 | VAL | PHE | ASP | LYS | ASP | GLY | ASN | GLY | TYR | ILE | ||||
| 11 | SER | ALA | ALA | GLU | LEU | ARG | HIS | VAL | MET | THR | ||||
| 12 | ASN | LEU | GLY | GLU | LYS | LEU | THR | ASP | GLU | GLU | ||||
| 13 | VAL | ASP | GLU | MET | ILE | ARG | GLU | ALA | ASP | ILE | ||||
| 14 | ASP | GLY | ASP | GLY | GLN | VAL | ASN | TYR | GLU | GLU | ||||
| 15 | PHE | VAL | GLN | MET | MET | THR | ALA | LYS |
Entity 2, SL3 Enhancer Factor2-1 one 21 residues - 2500 Da.
| 1 | LYS | GLU | ARG | ARG | MET | ALA | ASN | ASN | ALA | ARG | ||||
| 2 | GLU | ARG | LEU | ARG | VAL | ARG | GLY | GLY | CYS | GLY | ||||
| 3 | TRP |
Entity 3, CALCIUM ION, I - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_one: calmodulin, [U-13C; U-15N], 1.0 mM; SL3 Enhancer Factor2-1 1.0 mM; CALCIUM (II) ION 8.0 mM
sample_two: calmodulin, [U-15N], 1.0 mM; SL3 Enhancer Factor2-1 1.0 mM; CALCIUM (II) ION 8.0 mM
sample_conditions_one: pH: 6.0; temperature: 306 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| F1, F2 (13C/15N) filtered 1H-1H NOESY | sample_two | not available | sample_conditions_one |
| F1 (13C/15N) filtered 1H-1H TOCSY | sample_two | not available | sample_conditions_one |
| 1H-13C CT-HSQC | sample_two | not available | sample_conditions_one |
| (HB)CB(CGCD)HD | sample_two | not available | sample_conditions_one |
| CBCANH | sample_two | not available | sample_conditions_one |
| CBCACO(N)H | sample_two | not available | sample_conditions_one |
| HNCO | sample_two | not available | sample_conditions_one |
| HBHACO(N)H | sample_two | not available | sample_conditions_one |
| CTSL-HCANH | sample_two | not available | sample_conditions_one |
| 3D 1H-1H-15N NOESY | sample_two | not available | sample_conditions_one |
| 3D 1H-1H-13C NOESY | sample_two | not available | sample_conditions_one |
| (H)C(C)(CO)NH TOCSY | sample_two | not available | sample_conditions_one |
| H(CC)(CO)NH TOCSY | sample_two | not available | sample_conditions_one |
Software:
TRIPOS - data analysis
XWINNMR - processing
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker AMX-2 500 MHz
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4270 4284 |
| PDB | |
| DBJ | BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 |
| EMBL | CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 |
| GB | AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 |
| PIR | JC1305 MCON |
| PRF | 0409298A 0608335A |
| REF | NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 |
| SP | O02367 O16305 O96081 P02594 P05932 |
| TPG | DAA13808 DAA18029 DAA19590 DAA24777 DAA24988 |
| AlphaFold | O02367 O16305 O96081 P02594 P05932 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks