BMRB Entry 5068

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5068

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Title:
Recombinant hen lysozyme containing the extra N-terminal Met as the standard reference for the study of hen lysozyme variants
Deposition date:
2001-07-04
Original release date:
2003-12-22
Authors:
Noda, Yasuo; Yokota, Atsushi; Horii, Daisuke; Tominaga, Takeshi; Tanisaka, Yoshiaki; Tachibana, Hideki; Segawa, Shin-ichi
Citation:

Citation: Noda, Yasuo; Yokota, Atsushi; Horii, Daisuke; Tominaga, Takeshi; Tanisaka, Yoshiaki; Tachibana, Hideki; Segawa, Shin-ichi. "NMR Structural Study of Two-disulfide Variant of hen Lysozyme: 2SS[6-127, 30-115]--A Disulfide Intermediate with a Partly Unfolded Structure"  Biochemistry 41, 2130-2139 (2002).
PubMed: 11841203

Assembly members:

Assembly members:
hen egg white lysozyme, polymer, 130 residues, 14436 Da.

Natural source:

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hen egg white lysozyme: MKVFGRCELAAAMKRHGLDN YRGYSLGNWVCAAKFESNFN TQATNRNTDGSTDYGILQIN SRWWCNDGRTPGSRNLCNIP CSALLSSDITASVNCAKKIV SDGNGMNAWVAWRNRCKGTD VQAWIRGCRL

Data sets:
Data typeCount
1H chemical shifts788

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hen lysozyme1

Entities:

Entity 1, hen lysozyme 130 residues - 14436 Da.

1   METLYSVALPHEGLYARGCYSGLULEUALA
2   ALAALAMETLYSARGHISGLYLEUASPASN
3   TYRARGGLYTYRSERLEUGLYASNTRPVAL
4   CYSALAALALYSPHEGLUSERASNPHEASN
5   THRGLNALATHRASNARGASNTHRASPGLY
6   SERTHRASPTYRGLYILELEUGLNILEASN
7   SERARGTRPTRPCYSASNASPGLYARGTHR
8   PROGLYSERARGASNLEUCYSASNILEPRO
9   CYSSERALALEULEUSERSERASPILETHR
10   ALASERVALASNCYSALALYSLYSILEVAL
11   SERASPGLYASNGLYMETASNALATRPVAL
12   ALATRPARGASNARGCYSLYSGLYTHRASP
13   VALGLNALATRPILEARGGLYCYSARGLEU

Samples:

sample_1: hen egg white lysozyme, [U-15N], 1.0 – 1.2 mM

EX-cond_1: pH: 3.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
HOHAHAnot availablenot availablenot available
15N 3D-NOESY-HSQCnot availablenot availablenot available
15N 3D-TOCSY-HSQCnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available

Software:

xwinnmr v2.6 -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 1093 1104 1105 11052 1650 1651 1653 1770 1772 1798 1799 1800 1801 1802 1803 18304 18305 1865 1881 1882 1883 1884 1975 1977 2231 2446 2786 2858 2917 2957 4562 4831 4943 5069 568 569 5803 5804 6415 7143 7144 7159 7160 791
PDB
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AlphaFold P00698 P00699 P00700 Q7LZP9 Q7LZQ0