BMRB Entry 18304

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18304

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Title:
Order parameters for HEWL
Deposition date:
2012-02-29
Original release date:
2012-06-13
Authors:
Moorman, Veronica; Valentine, Kathleen; Wand, Joshua
Citation:

Citation: Moorman, Veronica; Valentine, Kathleen; Wand, A. Joshua. "The dynamical response of hen egg white lysozyme to the binding of a carbohydrate ligand."  Protein Sci. 21, 1066-1073 (2012).
PubMed: 22593013

Assembly members:

Assembly members:
HEWL, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HEWL: KVFGRCELAAAMKRHGLDNY RGYSLGNWVCAAKFESNFNT QATNRNTDGSTDYGILQINS RWWCNDGRTPGSRNLCNIPC SALLSSDITASVNCAKKIVS DGNGMNAWVAWRNRCKGTDV QAWIRGCRL

Data typeCount
order parameters174

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HEWL1

Entities:

Entity 1, HEWL 129 residues - Formula weight is not available

1   LYSVALPHEGLYARGCYSGLULEUALAALA
2   ALAMETLYSARGHISGLYLEUASPASNTYR
3   ARGGLYTYRSERLEUGLYASNTRPVALCYS
4   ALAALALYSPHEGLUSERASNPHEASNTHR
5   GLNALATHRASNARGASNTHRASPGLYSER
6   THRASPTYRGLYILELEUGLNILEASNSER
7   ARGTRPTRPCYSASNASPGLYARGTHRPRO
8   GLYSERARGASNLEUCYSASNILEPROCYS
9   SERALALEULEUSERSERASPILETHRALA
10   SERVALASNCYSALALYSLYSILEVALSER
11   ASPGLYASNGLYMETASNALATRPVALALA
12   TRPARGASNARGCYSLYSGLYTHRASPVAL
13   GLNALATRPILEARGGLYCYSARGLEU

Samples:

sample_1: HEWL, [U-100% 13C; U-100% 15N; U-55% 2H], 410 uM; acetate 100 mM; sodium azide 0.04%; H2O 90%; D2O 10%

sample_conditions_1: temperature: 308.15 K; pH: 4.7; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
N T1 relaxationsample_1isotropicsample_conditions_1
N T1 relaxationsample_1isotropicsample_conditions_1
N T2 relaxationsample_1isotropicsample_conditions_1
N T2 relaxationsample_1isotropicsample_conditions_1
N NOE relaxationsample_1isotropicsample_conditions_1
N NOE relaxationsample_1isotropicsample_conditions_1
IzCz relaxationsample_1isotropicsample_conditions_1
IzCz relaxationsample_1isotropicsample_conditions_1
IzCzDz relaxationsample_1isotropicsample_conditions_1
IzCzDz relaxationsample_1isotropicsample_conditions_1
IzCzDy relaxationsample_1isotropicsample_conditions_1
IzCzDy relaxationsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

Felix, Accelrys Software Inc. - processing

in-house_software, wandlab.org - data analysis

NMR spectrometers:

  • Bruker Avance 750 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 1093 1104 1105 11052 1650 1651 1653 1770 1772 1798 1799 1800 1801 1802 1803 18305 1865 1881 1882 1883 1884 1975 1977 2231 2446 2786 2858 2917 2957 4562
PDB
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AlphaFold Q7LZP9 P00698 P00699 P00700 Q7LZQ0