BMRB Entry 4943
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4943
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Citation: Poznanski, Jaroslaw; Georgalis, Yannis; Wehr, Lorin; Zielenkiewicz, Piotr; Saenger, Wolfram. "Comparison of Two Different Lysozyme Types Under Native and Crystallization
Conditions using Two-Dimensional NMR and Dynamic Light Scattering
" Biophys. Chem. ., .-. (2002).
Assembly members:
Chicken Egg White Lysozyme, polymer, 133 residues, Formula weight is not available
Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: vendor
Entity Sequences (FASTA):
Chicken Egg White Lysozyme: KVFGRCELAAAMKRHGLDNY
RGYSLGNWVCAAKFESNFNT
QATNRNTDGSTDYGILQINS
RWWCNDGRTPGSRNLCNIPC
SALLSSDITASVNCAKKIVS
DGNGMNAWVAWRNRCKGTDV
QAWIRGCRLDRSR
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | lysozyme | 1 |
Entities:
Entity 1, lysozyme 133 residues - Formula weight is not available
| 1 | LYS | VAL | PHE | GLY | ARG | CYS | GLU | LEU | ALA | ALA | ||||
| 2 | ALA | MET | LYS | ARG | HIS | GLY | LEU | ASP | ASN | TYR | ||||
| 3 | ARG | GLY | TYR | SER | LEU | GLY | ASN | TRP | VAL | CYS | ||||
| 4 | ALA | ALA | LYS | PHE | GLU | SER | ASN | PHE | ASN | THR | ||||
| 5 | GLN | ALA | THR | ASN | ARG | ASN | THR | ASP | GLY | SER | ||||
| 6 | THR | ASP | TYR | GLY | ILE | LEU | GLN | ILE | ASN | SER | ||||
| 7 | ARG | TRP | TRP | CYS | ASN | ASP | GLY | ARG | THR | PRO | ||||
| 8 | GLY | SER | ARG | ASN | LEU | CYS | ASN | ILE | PRO | CYS | ||||
| 9 | SER | ALA | LEU | LEU | SER | SER | ASP | ILE | THR | ALA | ||||
| 10 | SER | VAL | ASN | CYS | ALA | LYS | LYS | ILE | VAL | SER | ||||
| 11 | ASP | GLY | ASN | GLY | MET | ASN | ALA | TRP | VAL | ALA | ||||
| 12 | TRP | ARG | ASN | ARG | CYS | LYS | GLY | THR | ASP | VAL | ||||
| 13 | GLN | ALA | TRP | ILE | ARG | GLY | CYS | ARG | LEU | ASP | ||||
| 14 | ARG | SER | ARG |
Samples:
sample_A: Chicken Egg White Lysozyme 10 mM
sample_B: Chicken Egg White Lysozyme 10 mM
cond: pH: 3.8; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H TOCSY | not available | not available | cond |
| 1H-1H NOESY | not available | not available | cond |
Software:
nmrpipe v1.7 - data processing
XEasy v1.3.11 - peak assignment, sequential assignment
NMR spectrometers:
- Varian Unity Plus 500 MHz