BMRB Entry 5069

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5069

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Title:
NMR Structural Study of Two-Disulfide Variant of hen Lysozyme: 2SS[6-127, 30-115]-A Disulfide Intermediate with a Partly Unfolded Structure
Deposition date:
2001-07-06
Original release date:
2003-05-22
Authors:
Noda, Yasuo; Yokota, Atsushi; Horii, Daisuke; Tominaga, Takeshi; Tanisaka, Yoshiaki; Tachibana, Hideki; Segawa, Shin-ichi
Citation:

Citation: Noda, Yasuo; Yokota, Atsushi; Horii, Daisuke; Tominaga, Takeshi; Tanisaka, Yoshiaki; Tachibana, Hideki; Segawa, Shin-ichi. "NMR Structural Study of Two-disulfide Variant of hen Lysozyme : 2SS[6-127, 30-115]--A Disulfide Intermediate with a Partly Unfolded Structure"  Biochemistry 41, 2130-2139 (2002).
PubMed: 11841203

Assembly members:

Assembly members:
hen egg white lysozyme variant, polymer, 130 residues, 14311 Da.

Natural source:

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hen egg white lysozyme variant: MKVFGRCELAAAMKRHGLDN YRGYSLGNWVCAAKFESNFN TQATNRNTDGSTDYGILQIN SRWWANDGRTPGSRNLANIP ASALLSSDITASVNAAKKIV SDGNGMNAWVAWRNRCKGTD VQAWIRGCRL

Data sets:
Data typeCount
1H chemical shifts593

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hen lysozyme CYS mutants (64, 76, 80 and 94) by Ala1

Entities:

Entity 1, hen lysozyme CYS mutants (64, 76, 80 and 94) by Ala 130 residues - 14311 Da.

1   METLYSVALPHEGLYARGCYSGLULEUALA
2   ALAALAMETLYSARGHISGLYLEUASPASN
3   TYRARGGLYTYRSERLEUGLYASNTRPVAL
4   CYSALAALALYSPHEGLUSERASNPHEASN
5   THRGLNALATHRASNARGASNTHRASPGLY
6   SERTHRASPTYRGLYILELEUGLNILEASN
7   SERARGTRPTRPALAASNASPGLYARGTHR
8   PROGLYSERARGASNLEUALAASNILEPRO
9   ALASERALALEULEUSERSERASPILETHR
10   ALASERVALASNALAALALYSLYSILEVAL
11   SERASPGLYASNGLYMETASNALATRPVAL
12   ALATRPARGASNARGCYSLYSGLYTHRASP
13   VALGLNALATRPILEARGGLYCYSARGLEU

Samples:

sample_1: hen egg white lysozyme variant, [U-15N], 1.0 – 1.2 mM

EX-cond_1: pH: 3.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
HOHAHAnot availablenot availablenot available
15N 3D-NOESY-HSQCnot availablenot availablenot available
15N 3D-TOCSY-HSQCnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available

Software:

xwinnmr v2.6 -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 1093 11051 11052 11459 11460 15198 18304 18305 18365 4562 4943 5068 5803 5804 7135 7159 7160
PDB
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AlphaFold P00698