BMRB Entry 5803

Name: Three-disulfide variant of hen lysozyme: C64A/C80A
Published: 2004-09-14

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5803

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Three-disulfide variant of hen lysozyme: C64A/C80A

Deposition date:

2003-05-19

Original release date:

2004-09-14

Authors:

Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi

Citation:

Citation: Yokota, Atsushi; Hirai, Kenichi; Miyauchi, Hiroyo; Iimura, Satoshi; Noda, Yasuo; Inoue, Kyouko; Akasaka, Kazuyuki; Tachibana, Hideki; Segawa, Shin-ichi. "NMR characterization of three-disulfide variants of lysozyme, C64A/C80A, C76A/C94A, and C30A/C115A--a marginally stable state in folded proteins." Biochemistry 43, 6663-6669 (2004).
PubMed: 15157100

Assembly members:

Assembly members:
three-disulfide variant of hen lysozyme, polymer, 130 residues, 14374 Da.

Natural source:

Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
three-disulfide variant of hen lysozyme: MKVFGRCELAAAMKRHGLDN YRGYSLGNWVCAAKFESNFN TQATNRNTDGSTDYGILQIN SRWWANDGRTPGSRNLCNIP ASALLSSDITASVNCAKKIV SDGNGMNAWVAWRNRCKGTD VQAWIRGCRL

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	680

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hen lysozyme Cys mutants, C64A/C80A	1

Entities:

Entity 1, hen lysozyme Cys mutants, C64A/C80A 130 residues - 14374 Da.

1	MET	LYS	VAL	PHE	GLY	ARG	CYS	GLU	LEU	ALA
2	ALA	ALA	MET	LYS	ARG	HIS	GLY	LEU	ASP	ASN
3	TYR	ARG	GLY	TYR	SER	LEU	GLY	ASN	TRP	VAL
4	CYS	ALA	ALA	LYS	PHE	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	THR	ASN	ARG	ASN	THR	ASP	GLY
6	SER	THR	ASP	TYR	GLY	ILE	LEU	GLN	ILE	ASN
7	SER	ARG	TRP	TRP	ALA	ASN	ASP	GLY	ARG	THR
8	PRO	GLY	SER	ARG	ASN	LEU	CYS	ASN	ILE	PRO
9	ALA	SER	ALA	LEU	LEU	SER	SER	ASP	ILE	THR
10	ALA	SER	VAL	ASN	CYS	ALA	LYS	LYS	ILE	VAL
11	SER	ASP	GLY	ASN	GLY	MET	ASN	ALA	TRP	VAL
12	ALA	TRP	ARG	ASN	ARG	CYS	LYS	GLY	THR	ASP
13	VAL	GLN	ALA	TRP	ILE	ARG	GLY	CYS	ARG	LEU

Samples:

sample_1: three-disulfide variant of hen lysozyme, [U-15N], 0.3 – 1.0 mM

EX-cond_1: pH: 3.8 na; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
DQF-COSY	not available	not available	not available
NOESY	not available	not available	not available
HOHAHA	not available	not available	not available
15N 3D-NOESY-HSQC	not available	not available	not available
15N 3D-TOCSY-HSQC	not available	not available	not available
1H-15N HSQC	not available	not available	not available
15N 3D-HSQC-NOESY-HSQC	not available	not available	not available

Software:

XWINNMR v2.6 -

NMR spectrometers:

Bruker DRX 600 MHz

Related Database Links:

SWISS-PROT	P00698
GenBank	AAL69327 AAA48943
PDB	8LYZ 7LYZ 6LYZ 6LYT 5LYZ 5LYT 5LYM 4LZT 4LYZ 4LYT 4LYO 4LYM 3LZT 3LYZ 3LYT 3LYO 3LYM 3HFM 3D9A 3B6L 2Z19 2Z18 2Z12 2YVB 2YSS 2VB1 2Q0M 2PC2 2LZT 2LZH 2LYZ 2LYO 2LYM 2I6Z 2I26 2I25 2HUB 2HU3 2HU1 2HTX 2HSO 2HS9 2HS7 2H9K 2H9J 2G4Q 2G4P 2FBB 2F4G 2F4A 2F30 2F2N 2EPE 2EKS 2EIZ 2DQJ 2DQI 2DQH 2DQG 2DQF 2DQE 2DQD 2DQC 2D91 2D6B 2D4K 2D4J 2D4I 2CGI 2CDS 2C8P 2C8O 2BPU 2BLY 2BLX 2B5Z 2AUB 2A7F 2A7D 2A6U 1ZVY 1ZVH 1ZV5 1ZMY 1Z55 1YQV 1YL1 1YL0 1YKZ 1YKY 1YKX 1YIL 1YIK 1XGU 1XGT 1XGR 1XGQ 1XGP 1XFP 1XEK 1XEJ 1XEI 1WTN 1WTM 1W6Z 1VFB 1VED 1VDT 1VDS 1VDQ 1VDP 1VAU 1VAT 1V7T 1V7S 1UUZ 1UIH 1UIG 1UCO 1UC0 1UA6 1T6V 1T3P 1SQ2 1SFG 1SFB 1SF7 1SF6 1SF4 1RJC 1RI8 1RFP 1RCM 1QTK 1QIO 1PS5 1P2C 1NDM 1N4F 1MLC 1MEL 1LZT 1LZN 1LZH 1LZC 1LZB 1LZA 1LZ9 1LZ8 1LYZ 1LYS 1LYO 1LSG 1LSF 1LSE 1LSD 1LSC 1LSB 1LSA 1LPI 1LMA 1LKS 1LKR 1LJK 1LJJ 1LJI 1LJH 1LJG 1LJF 1LJE 1LJ4 1LJ3 1LCN 1KIR 1KIQ 1KIP 1JTT 1JTO 1JPO 1JJ3 1JJ1 1JJ0 1JIY 1JIT 1JIS 1JA7 1JA6 1JA4 1JA2 1J1X 1J1P 1J1O 1IO5 1IEE 1IC7 1IC5 1IC4 1HSX 1HSW 1HF4 1HEW 1HEL 1HC0 1H87 1GXX 1GXV 1GWD 1GPQ 1G7M 1G7L 1G7J 1G7I 1G7H 1FDL 1F10 1F0W 1E8L 1DQJ 1DPX 1DPW 1C10 1C08 1BWJ 1BWI 1BWH 1BVX 1BVK 1BHZ 1BGI 1B2K 1B0D 1AZF 1AKI 194L 193L
BMRB	5069 5068 4943 4562 1093

1	MET	LYS	VAL	PHE	GLY	ARG	CYS	GLU	LEU	ALA
2	ALA	ALA	MET	LYS	ARG	HIS	GLY	LEU	ASP	ASN
3	TYR	ARG	GLY	TYR	SER	LEU	GLY	ASN	TRP	VAL
4	CYS	ALA	ALA	LYS	PHE	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	THR	ASN	ARG	ASN	THR	ASP	GLY
6	SER	THR	ASP	TYR	GLY	ILE	LEU	GLN	ILE	ASN
7	SER	ARG	TRP	TRP	ALA	ASN	ASP	GLY	ARG	THR
8	PRO	GLY	SER	ARG	ASN	LEU	CYS	ASN	ILE	PRO
9	ALA	SER	ALA	LEU	LEU	SER	SER	ASP	ILE	THR
10	ALA	SER	VAL	ASN	CYS	ALA	LYS	LYS	ILE	VAL
11	SER	ASP	GLY	ASN	GLY	MET	ASN	ALA	TRP	VAL
12	ALA	TRP	ARG	ASN	ARG	CYS	LYS	GLY	THR	ASP
13	VAL	GLN	ALA	TRP	ILE	ARG	GLY	CYS	ARG	LEU

1	MET	LYS	VAL	PHE	GLY	ARG	CYS	GLU	LEU	ALA
2	ALA	ALA	MET	LYS	ARG	HIS	GLY	LEU	ASP	ASN
3	TYR	ARG	GLY	TYR	SER	LEU	GLY	ASN	TRP	VAL
4	CYS	ALA	ALA	LYS	PHE	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	THR	ASN	ARG	ASN	THR	ASP	GLY
6	SER	THR	ASP	TYR	GLY	ILE	LEU	GLN	ILE	ASN
7	SER	ARG	TRP	TRP	ALA	ASN	ASP	GLY	ARG	THR
8	PRO	GLY	SER	ARG	ASN	LEU	CYS	ASN	ILE	PRO
9	ALA	SER	ALA	LEU	LEU	SER	SER	ASP	ILE	THR
10	ALA	SER	VAL	ASN	CYS	ALA	LYS	LYS	ILE	VAL
11	SER	ASP	GLY	ASN	GLY	MET	ASN	ALA	TRP	VAL
12	ALA	TRP	ARG	ASN	ARG	CYS	LYS	GLY	THR	ASP
13	VAL	GLN	ALA	TRP	ILE	ARG	GLY	CYS	ARG	LEU

1	MET	LYS	VAL	PHE	GLY	ARG	CYS	GLU	LEU	ALA
2	ALA	ALA	MET	LYS	ARG	HIS	GLY	LEU	ASP	ASN
3	TYR	ARG	GLY	TYR	SER	LEU	GLY	ASN	TRP	VAL
4	CYS	ALA	ALA	LYS	PHE	GLU	SER	ASN	PHE	ASN
5	THR	GLN	ALA	THR	ASN	ARG	ASN	THR	ASP	GLY
6	SER	THR	ASP	TYR	GLY	ILE	LEU	GLN	ILE	ASN
7	SER	ARG	TRP	TRP	ALA	ASN	ASP	GLY	ARG	THR
8	PRO	GLY	SER	ARG	ASN	LEU	CYS	ASN	ILE	PRO
9	ALA	SER	ALA	LEU	LEU	SER	SER	ASP	ILE	THR
10	ALA	SER	VAL	ASN	CYS	ALA	LYS	LYS	ILE	VAL
11	SER	ASP	GLY	ASN	GLY	MET	ASN	ALA	TRP	VAL
12	ALA	TRP	ARG	ASN	ARG	CYS	LYS	GLY	THR	ASP
13	VAL	GLN	ALA	TRP	ILE	ARG	GLY	CYS	ARG	LEU