BMRB Entry 16130

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16130

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for colicinN Tdomain residues 40-76 in complex with unlabbeled TolAIII
Deposition date:
2009-01-23
Original release date:
2009-03-30
Authors:
Hecht, Oliver; Moore, Geoffrey
Citation:

Citation: Hecht, Oliver; Ridley, Helen; Lakey, Jeremy; Moore, Geoffrey. "A common interaction for the entry of colicin N and filamentous phage into Escherichia Coli"  J. Mol. Biol. 388, 880-893 (2009).
PubMed: 19306883

Assembly members:

Assembly members:
Tdom40-76, polymer, 90 residues, Formula weight is not available
TolAIII, polymer, 135 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: ptoletdom

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tdom40-76: MGSNGADNAHNNAFGGGKNP GIGNTSGAGSNGSASSNRGN SNGWSWSNKPHKNDGFHSDG SYHITFHGDNNSKPKPGGNS GNRGNNGDGA
TolAIII: HHHHHHSSQIFSVTLSSGKN APKTGGGAKGNNASPAGSGN TKNNGASGADINNYAGQIKS AIESKFYDASSYAGKTCTLR IKLAPDGMLLDIKPEGGDPA LCQAALAAAKLAKIPKPPSQ AVYEVFKNAPLDFKP

Data sets:
Data typeCount
13C chemical shifts92
15N chemical shifts29
1H chemical shifts29

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tdom40-761
2TolAIII2

Entities:

Entity 1, Tdom40-76 90 residues - Formula weight is not available

1   METGLYSERASNGLYALAASPASNALAHIS
2   ASNASNALAPHEGLYGLYGLYLYSASNPRO
3   GLYILEGLYASNTHRSERGLYALAGLYSER
4   ASNGLYSERALASERSERASNARGGLYASN
5   SERASNGLYTRPSERTRPSERASNLYSPRO
6   HISLYSASNASPGLYPHEHISSERASPGLY
7   SERTYRHISILETHRPHEHISGLYASPASN
8   ASNSERLYSPROLYSPROGLYGLYASNSER
9   GLYASNARGGLYASNASNGLYASPGLYALA

Entity 2, TolAIII 135 residues - Formula weight is not available

1   HISHISHISHISHISHISSERSERGLNILE
2   PHESERVALTHRLEUSERSERGLYLYSASN
3   ALAPROLYSTHRGLYGLYGLYALALYSGLY
4   ASNASNALASERPROALAGLYSERGLYASN
5   THRLYSASNASNGLYALASERGLYALAASP
6   ILEASNASNTYRALAGLYGLNILELYSSER
7   ALAILEGLUSERLYSPHETYRASPALASER
8   SERTYRALAGLYLYSTHRCYSTHRLEUARG
9   ILELYSLEUALAPROASPGLYMETLEULEU
10   ASPILELYSPROGLUGLYGLYASPPROALA
11   LEUCYSGLNALAALALEUALAALAALALYS
12   LEUALALYSILEPROLYSPROPROSERGLN
13   ALAVALTYRGLUVALPHELYSASNALAPRO
14   LEUASPPHELYSPRO

Samples:

Tdom40-76+tola: Tdom40-76, [U-100% 13C; U-100% 15N], 1.2 mM; H2O 90%; D2O 10%; TolAIII 1.2 mM

sample_conditions_1: ionic strength: 0.35 M; pH: 6.8; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCTdom40-76+tolaisotropicsample_conditions_1
3D HNCOTdom40-76+tolaisotropicsample_conditions_1
3D CBCA(CO)NHTdom40-76+tolaisotropicsample_conditions_1
3D HNCACBTdom40-76+tolaisotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15506 16131 16133 16134 4771
EMBL CAA68592 CTB58308
GB EMX27764 EMZ96444 KHH25654 KHH41883 KHH74941 AHA63696 AHG07456 AHY69283 EGB67246 EGC08283
REF WP_001749638 WP_004026235 WP_024139131 WP_032488335 WP_040089861 WP_000030617 WP_000030621 WP_000030643 WP_000030697 WP_000030705
SP P08083
PDB
AlphaFold P08083

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks