BMRB Entry 16134

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16134

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for tolaIII residues296-421in complex with unlabelled colicin N Tdomain
Deposition date:
2009-01-23
Original release date:
2009-04-17
Authors:
Hecht, Oliver; Moore, Geoffrey
Citation:

Citation: Hecht, Oliver; Ridley, Helen; Lakey, Jeremy; Moore, Geoffrey. "A common interation for the entry of colicin N and filamentous phage into Escherichia Coli"  J. Mol. Biol. 388, 880-893 (2009).
PubMed: 19306883

Assembly members:

Assembly members:
TolAIII, polymer, 135 residues, Formula weight is not available
Tdom40-76, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet8c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TolAIII: HHHHHHSSQIFSVTLSSGKN APKTGGGAKGNNASPAGSGN TKNNGASGADINNYAGQIKS AIESKFYDASSYAGKTCTLR IKLAPDGMLLDIKPEGGDPA LCQAALAAAKLAKIPKPPSQ AVYEVFKNAPLDFKP
Tdom40-76: MGSNGADNAHNNAFGGGKNP GIGNTSGAGSNGSASSNRGN SNGWSWSNKPHKNDGFHSDG SYHITFHGDNNSKPKPGGNS GNRGNNGDGA

Data sets:
Data typeCount
13C chemical shifts148
15N chemical shifts71
1H chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TolAIII1
2Tdom40-762

Entities:

Entity 1, TolAIII 135 residues - Formula weight is not available

1   HISHISHISHISHISHISSERSERGLNILE
2   PHESERVALTHRLEUSERSERGLYLYSASN
3   ALAPROLYSTHRGLYGLYGLYALALYSGLY
4   ASNASNALASERPROALAGLYSERGLYASN
5   THRLYSASNASNGLYALASERGLYALAASP
6   ILEASNASNTYRALAGLYGLNILELYSSER
7   ALAILEGLUSERLYSPHETYRASPALASER
8   SERTYRALAGLYLYSTHRCYSTHRLEUARG
9   ILELYSLEUALAPROASPGLYMETLEULEU
10   ASPILELYSPROGLUGLYGLYASPPROALA
11   LEUCYSGLNALAALALEUALAALAALALYS
12   LEUALALYSILEPROLYSPROPROSERGLN
13   ALAVALTYRGLUVALPHELYSASNALAPRO
14   LEUASPPHELYSPRO

Entity 2, Tdom40-76 90 residues - Formula weight is not available

1   METGLYSERASNGLYALAASPASNALAHIS
2   ASNASNALAPHEGLYGLYGLYLYSASNPRO
3   GLYILEGLYASNTHRSERGLYALAGLYSER
4   ASNGLYSERALASERSERASNARGGLYASN
5   SERASNGLYTRPSERTRPSERASNLYSPRO
6   HISLYSASNASPGLYPHEHISSERASPGLY
7   SERTYRHISILETHRPHEHISGLYASPASN
8   ASNSERLYSPROLYSPROGLYGLYASNSER
9   GLYASNARGGLYASNASNGLYASPGLYALA

Samples:

sample_1: TolAIII, [U-100% 13C; U-100% 15N], 0.8 mM; Tdom40-76 0.8 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.35 M; pH: 6.8; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16133 15506 16130 16131
PDB
EMBL CTW32328 CAA68592
GB AHA63696 AHG07456 AHY69283 EGB67246 EGC08283 EMX27764 EMZ96444 KHH25654 KHH41883 KHH74941
REF WP_000030617 WP_000030621 WP_000030643 WP_000030697 WP_000030705 WP_001749638 WP_004026235 WP_024139131 WP_032488335 YP_009073784
SP P08083
AlphaFold P08083

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks