BMRB Entry 15506

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the intrinsically disordered translocation domain of colicin N
Deposition date:
2007-10-04
Original release date:
2008-06-26
Authors:
Hecht, Oliver; Moore, Geoff
Citation:

Citation: Hecht, O.; Ridley, H.; Boetzel, R.; Lewin, A.; Cull, N.; Chalton, D.; Lakey, J.; Moore, G.. "Self-recognition by an intrinsically disordered protein."  FEBS Lett. 582, 2673-2677 (2008).
PubMed: 18573254

Assembly members:

Assembly members:
Colicin_N_T_domain, polymer, 90 residues, 8924 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pOHN1

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts238
15N chemical shifts84
1H chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Colicin_N_T_domain1

Entities:

Entity 1, Colicin_N_T_domain 90 residues - 8924 Da.

1   GLYSERASNGLYALAASPASNALAHISASN
2   ASNALAPHEGLYGLYGLYLYSASNPROGLY
3   ILEGLYASNTHRSERGLYALAGLYSERASN
4   GLYSERALASERSERASNARGGLYASNSER
5   ASNGLYTRPSERTRPSERASNLYSPROHIS
6   LYSASNASPGLYPHEHISSERASPGLYSER
7   TYRHISILETHRPHEHISGLYASPASNASN
8   SERLYSPROLYSPROGLYGLYASNSERGLY
9   ASNARGGLYASNASNGLYASPGLYALASER

Samples:

sample_1: Colicin N T domain, [U-100% 13C; U-100% 15N], 1.1 mM; sodium azide 0.01%; sodium phosphate 50 mM; sodium chloride 300 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.35 M; pH: 6.8; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
HNNsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

BMRB 16130 16131
EMBL CAA68592 CTB58308
GB EMX27764 EMZ96444 KHH25654 KHH41883 KHH74941
REF WP_001749638 WP_004026235 WP_024139131 WP_032488335 WP_040089861
SP P08083
AlphaFold P08083

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks