BMRB Entry 16133

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for TolAIII residues 296-421
Published: 2009-04-22

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16133

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for TolAIII residues 296-421

Deposition date:

2009-01-23

Original release date:

2009-04-22

Authors:

Hecht, Oliver

Citation:

Citation: Hecht, Oliver; Ridley, Helen; Lakey, Jeremy; Moore, Geoffrey. "A common interaction for the entry of colicin N and filamentous phage into Escherichia coli" J. Mol. Biol. 388, 880-893 (2009).
PubMed: 19306883

Assembly members:

Assembly members:
TolAIII, polymer, 135 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet8c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TolAIII: HHHHHHSSQIFSVTLSSGKN APKTGGGAKGNNASPAGSGN TKNNGASGADINNYAGQIKS AIESKFYDASSYAGKTCTLR IKLAPDGMLLDIKPEGGDPA LCQAALAAAKLAKIPKPPSQ AVYEVFKNAPLDFKP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	145
15N chemical shifts	77
1H chemical shifts	77

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TolAIII	1

Entities:

Entity 1, TolAIII 135 residues - Formula weight is not available

1

HIS

SER

GLN

ILE

2

PHE

SER

VAL

THR

LEU

SER

GLY

LYS

ASN

3

ALA

PRO

LYS

THR

GLY

ALA

LYS

GLY

4

ASN

ALA

SER

PRO

ALA

GLY

SER

GLY

ASN

5

THR

LYS

ASN

GLY

ALA

SER

GLY

ALA

ASP

6

ILE

ASN

TYR

ALA

GLY

GLN

ILE

LYS

SER

7

ALA

ILE

GLU

SER

LYS

PHE

TYR

ASP

ALA

SER

8

SER

TYR

ALA

GLY

LYS

THR

CYS

THR

LEU

ARG

9

ILE

LYS

LEU

ALA

PRO

ASP

GLY

MET

LEU

10

ASP

ILE

LYS

PRO

GLU

GLY

ASP

PRO

ALA

11

LEU

CYS

GLN

ALA

LEU

ALA

LYS

12

LEU

ALA

LYS

ILE

PRO

LYS

PRO

SER

GLN

13

ALA

VAL

TYR

GLU

VAL

PHE

LYS

ASN

ALA

PRO

14

LEU

ASP

PHE

LYS

PRO

Samples:

sample_1: TolAIII, [U-100% 13C; U-100% 15N], 0.6 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.35 M; pH: 6.8; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 600 MHz

BMRB	16134
PDB	1S62 1TOL 2X9A
EMBL	CTW32328
GB	AHA63696 AHG07456 AHY69283 EGB67246 EGC08283
REF	WP_000030617 WP_000030621 WP_000030643 WP_000030697 WP_000030705