BMRB Entry 16131

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16131

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the Colicin N Tdomain residues 40-76
Deposition date:
2009-01-23
Original release date:
2009-03-30
Authors:
Moore, Geoffrey; Oliver, Hecht
Citation:

Citation: Hecht, Oliver; Ridley, Helen; Lakey, Jeremy; Moore, Geoffrey. "A common interaction for the entry of colicin N and filamentous phage into Escherichia coli"  J. Mol. Biol. 388, 880-893 (2009).
PubMed: 19306883

Assembly members:

Assembly members:
Tdom40-47, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: ptoletdom

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tdom40-47: MGSNGADNAHNNAFGGGKNP GIGNTSGAGSNGSASSNRGN SNGWSWSNKPHKNDGFHSDG SYHITFHGDNNSKPKPGGNS GNRGNNGDGA

Data sets:
Data typeCount
13C chemical shifts104
15N chemical shifts35
1H chemical shifts35

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tdom40-761

Entities:

Entity 1, Tdom40-76 90 residues - Formula weight is not available

1   METGLYSERASNGLYALAASPASNALAHIS
2   ASNASNALAPHEGLYGLYGLYLYSASNPRO
3   GLYILEGLYASNTHRSERGLYALAGLYSER
4   ASNGLYSERALASERSERASNARGGLYASN
5   SERASNGLYTRPSERTRPSERASNLYSPRO
6   HISLYSASNASPGLYPHEHISSERASPGLY
7   SERTYRHISILETHRPHEHISGLYASPASN
8   ASNSERLYSPROLYSPROGLYGLYASNSER
9   GLYASNARGGLYASNASNGLYASPGLYALA

Samples:

sample_1: Tdom40-47, [U-100% 13C; U-100% 15N], 1.2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.35 M; pH: 6.8; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15506 16130
EMBL CAA68592 CTB58308
GB EMX27764 EMZ96444 KHH25654 KHH41883 KHH74941
REF WP_001749638 WP_004026235 WP_024139131 WP_032488335 WP_040089861
SP P08083
AlphaFold P08083

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks