BMRB Entry 4804

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4804

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Title:
1H,13C and 15N chemical shifts of bovine ferrous cytochrome b5-N17D
Deposition date:
2000-08-14
Original release date:
2000-11-15
Authors:
Hom, Kellie; Ma, Qi-Feng; Wolfe, Gary; Zhang, Hong; Storch, Elizabeth; Daggett, Valerie; Basus, Vladimir; Waskell, Lucy
Citation:

Citation: Hom, Kellie; Ma, Qi-Feng; Wolfe, Gary; Zhang, Hong; Storch, Elizabeth; Daggett, Valerie; Basus, Vladimir; Waskell, Lucy. "NMR Studies of the Association of Cytochrome b5 with Cytochrome c"  Biochemistry ., .-..

Assembly members:

Assembly members:
ferrous cytochrome b5, polymer, 82 residues, 9966 Da.
HEM, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pUC19

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ferrous cytochrome b5: AVKYYTLEEIQKHNDSKSTW LILHYKVYDLTKFLEEHPGG EEVLREQAGGDATENFEDVG HSTDARELSKTFIIGELHPD DR

Data sets:
Data typeCount
1H chemical shifts578
13C chemical shifts373
15N chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cytb5-N17D1
2heme2

Entities:

Entity 1, cytb5-N17D 82 residues - 9966 Da.

1   ALAVALLYSTYRTYRTHRLEUGLUGLUILE
2   GLNLYSHISASNASPSERLYSSERTHRTRP
3   LEUILELEUHISTYRLYSVALTYRASPLEU
4   THRLYSPHELEUGLUGLUHISPROGLYGLY
5   GLUGLUVALLEUARGGLUGLNALAGLYGLY
6   ASPALATHRGLUASNPHEGLUASPVALGLY
7   HISSERTHRASPALAARGGLULEUSERLYS
8   THRPHEILEILEGLYGLULEUHISPROASP
9   ASPARG

Entity 2, heme - C34 H32 N4 O4 FE1 - Formula weight is not available

1   HEM

Related Database Links:

BMRB 1323 1324 17457 4803 4806 4807 5745 6131
PDB 1CYO 1EHB 1ES1 1HKO 1J0Q 1LQX 1LR6 1M20 1M59 1NX7 1SH4 1U9M 1U9U 4HIN
EMBL CAA31949
GB AAB32285 AAC14455 AAC48779 AAC48780 AAI08114
PRF 1106188A 1106188C 1803548B
REF NP_001001770 NP_001159663 NP_001180227 NP_001274163 NP_776458
SP P00171 P00172
TPG DAA15965
AlphaFold P00171 P00172

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