BMRB Entry 4806
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4806
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Citation: Hom, Kellie; Ma, Qi-Feng; Wolfe, Gary; Zhang, Hong; Storch, Elizabeth; Daggett, Valerie; Basus, Vladimir; Waskell, Lucy. "NMR Studies of the Association of Cytochrome b5 with Cytochrome c" Biochemistry ., .-..
Assembly members:
ferric cytochrome b5, polymer, 82 residues, 9966 Da.
HEM, non-polymer, 616.487 Da.
Natural source: Common Name: bovine Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pUC19
Entity Sequences (FASTA):
ferric cytochrome b5: AVKYYTLEEIQKHNNSKSTW
LILHYKVYDLTKFLEEHPGG
EEVLREQAGGDATENFEDVG
HSTDARELSKTFIIGELHPD
DR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 132 |
| 13C chemical shifts | 90 |
| 15N chemical shifts | 76 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cytb5 | 1 |
| 2 | heme | 2 |
Entities:
Entity 1, cytb5 82 residues - 9966 Da.
| 1 | ALA | VAL | LYS | TYR | TYR | THR | LEU | GLU | GLU | ILE | ||||
| 2 | GLN | LYS | HIS | ASN | ASN | SER | LYS | SER | THR | TRP | ||||
| 3 | LEU | ILE | LEU | HIS | TYR | LYS | VAL | TYR | ASP | LEU | ||||
| 4 | THR | LYS | PHE | LEU | GLU | GLU | HIS | PRO | GLY | GLY | ||||
| 5 | GLU | GLU | VAL | LEU | ARG | GLU | GLN | ALA | GLY | GLY | ||||
| 6 | ASP | ALA | THR | GLU | ASN | PHE | GLU | ASP | VAL | GLY | ||||
| 7 | HIS | SER | THR | ASP | ALA | ARG | GLU | LEU | SER | LYS | ||||
| 8 | THR | PHE | ILE | ILE | GLY | GLU | LEU | HIS | PRO | ASP | ||||
| 9 | ASP | ARG |
Entity 2, heme - C34 H32 Fe N4 O4 - 616.487 Da.
| 1 | HEM |
Related Database Links:
Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone
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