BMRB Entry 4803

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4803

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Title:
1H,13C and 15N chemical shifts of bovine ferrous cytochrome b5
Deposition date:
2000-08-14
Original release date:
2000-11-15
Authors:
Hom, Kellie; Ma, Qi-Feng; Wolfe, Gary; Zhang, Hong; Storch, Elizabeth; Daggett, Valerie; Basus, Vladimir; Waskell, Lucy
Citation:

Citation: Hom, Kellie; Ma, Qi-Feng; Wolfe, Gary; Zhang, Hong; Storch, Elizabeth; Daggett, Valerie; Basus, Vladimir; Waskell, Lucy. "NMR Studies of the Association of Cytochrome b5 with Cytochrome c"  Biochemistry ., .-..

Assembly members:

Assembly members:
ferrous cytochrome b5, polymer, 82 residues, 9966 Da.
HEM, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pUC19

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ferrous cytochrome b5: AVKYYTLEEIQKHNNSKSTW LILHYKVYDLTKFLEEHPGG EEVLREQAGGDATENFEDVG HSTDARELSKTFIIGELHPD DR

Data sets:
Data typeCount
1H chemical shifts578
13C chemical shifts373
15N chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytb51
2heme2

Entities:

Entity 1, cytb5 82 residues - 9966 Da.

1   ALAVALLYSTYRTYRTHRLEUGLUGLUILE
2   GLNLYSHISASNASNSERLYSSERTHRTRP
3   LEUILELEUHISTYRLYSVALTYRASPLEU
4   THRLYSPHELEUGLUGLUHISPROGLYGLY
5   GLUGLUVALLEUARGGLUGLNALAGLYGLY
6   ASPALATHRGLUASNPHEGLUASPVALGLY
7   HISSERTHRASPALAARGGLULEUSERLYS
8   THRPHEILEILEGLYGLULEUHISPROASP
9   ASPARG

Entity 2, heme - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: ferrous cytochrome b5, [U-90% 13C; U-90% 15N], 2 – 9 mM; PROTOPORPHYRIN IX CONTAINING FE2 – 9 mM

ex-cond_1: pH*: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableex-cond_1
13C-1H HSQCsample_1not availableex-cond_1
(HB)CB(CGCD)HDsample_1not availableex-cond_1
(HB)CB(CGCDCE)HEsample_1not availableex-cond_1
1H-TOCSY-relayed ct-[13C, 1H]-HMQCsample_1not availableex-cond_1
HNCOsample_1not availableex-cond_1
HNCAsample_1not availableex-cond_1
HNCACBsample_1not availableex-cond_1
CBCA(CO)NNHsample_1not availableex-cond_1
HBHA(CO)NNHsample_1not availableex-cond_1
HCCH-TOCSYsample_1not availableex-cond_1
ct-HCACOsample_1not availableex-cond_1
13C-edited NOESY-HMQCsample_1not availableex-cond_1
15N-edited NOESY-HMQCsample_1not availableex-cond_1

Software:

Striker - FID transformation

nmrPipe - FID transformation

Sparky v3.3.2 - Spectral analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian Unity plus 600 MHz

Related Database Links:

BMRB 1323 1324 17457 4804 4806 4807 5745 6131
PDB
EMBL CAA31949
GB AAA72186 AAB32285 AAC14455 AAC48779 AAC48780
PRF 1106188A 1106188C 1803548B
REF NP_001001770 NP_001159663 NP_001180227 NP_001274163 NP_776458
SP P00171 P00172
TPG DAA15965
AlphaFold P00171 P00172

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks