BMRB Entry 5745

Title:
NMR Structure of bovine cytochrome b5
Deposition date:
2003-03-18
Original release date:
2007-11-05
Authors:
Muskett, Frederick; Whitford, David
Citation:

Citation: Muskett, Frederick; Whitford, David. "The solution structure of bovine ferricytochrome b5 determined using heteronuclear NMR methods"  J. Biomol. NMR 258, 172-189 (1996).
PubMed: 8613986

Assembly members:

Assembly members:
Cytochrome b5, polymer, 104 residues, 11795 Da.
HEM, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21d

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts508
13C chemical shifts307
15N chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome b5 monomer1
2HEM_ox2

Entities:

Entity 1, cytochrome b5 monomer 104 residues - 11795 Da.

1   ALAGLUGLUSERSERLYSALAVALLYSTYR
2   TYRTHRLEUGLUGLUILEGLNLYSHISASN
3   ASNSERLYSSERTHRTRPLEUILELEUHIS
4   TYRLYSVALTYRASPLEUTHRLYSPHELEU
5   GLUGLUHISPROGLYGLYGLUGLUVALLEU
6   ARGGLUGLNALAGLYGLYASPALATHRGLU
7   ASNPHEGLUASPVALGLYHISSERTHRASP
8   ALAARGGLULEUSERLYSTHRPHEILEILE
9   GLYGLULEUHISPROASPASPARGSERLYS
10   ILETHRLYSPROSERGLUSERILEILETHR
11   THRILEASPSER

Entity 2, HEM_ox - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: Cytochrome b5, [U-13C; U-15N], 1.0 – 2.0 mM; Phosphate 20 mM

Experimental_Conditions: pH: 7.0; temperature: 300 K; ionic strength: 0.08 M

Experiments:

NameSampleSample stateSample conditions
15H-1H HSQCsample_1not availableExperimental_Conditions
15H-1H TOCSY-HSQCsample_1not availableExperimental_Conditions
15H-1H NOESY-HSQCsample_1not availableExperimental_Conditions
HNCOsample_1not availableExperimental_Conditions
CBCANHsample_1not availableExperimental_Conditions
CBCA(CO)NHsample_1not availableExperimental_Conditions
HCCH-TOCSYsample_1not availableExperimental_Conditions
(HB)CB(CGCD)HD/HEsample_1not availableExperimental_Conditions
13C-edited NOESY-HSQCsample_1not availableExperimental_Conditions

Software:

XEASY v1.5 - Resonance assignment, NOE peak volume measurement

NMR spectrometers:

  • Varian Unity 600 MHz
  • Varian UnityPlus 500 MHz
  • Varian Inova 600 MHz

Related Database Links:

SWISS-PROT P00171
REF NP_776458
PRF 1803548B 1106188A
GenBank ABQ12619 AAI08114 AAC14455
EMBL CAA31949
PDB
BMRB 6131 4810 4809 4808 4807 4806 4805 4804 4803 294 1324 1323

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks