BMRB Entry 17457
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17457
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Citation: Muskett, Frederick; Kelly, Geoffrey; Whitford, David. "The Solution Structure of Bovine Ferricytochrome b5 Determined Using Heteronuclear NMR Methods" J. Mol. Biol. 258, 172-189 (1996).
Assembly members:
b5, polymer, 104 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.
Natural source: Common Name: cattle Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pKK223-3
Entity Sequences (FASTA):
b5: AEESSKAVKYYTLEEIQKHN
NSKSTWLILHYKVYDLTKFL
EEHPGGEEVLREQAGGDATE
NFEDVGHSTDARELSKTFII
GELHPDDRSKITKPSESIIT
TIDS
- chemical_rates
| Data type | Count |
| kinetic rates | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | b5 | 1 |
| 2 | HEM | 2 |
Entities:
Entity 1, b5 104 residues - Formula weight is not available
| 1 | ALA | GLU | GLU | SER | SER | LYS | ALA | VAL | LYS | TYR | ||||
| 2 | TYR | THR | LEU | GLU | GLU | ILE | GLN | LYS | HIS | ASN | ||||
| 3 | ASN | SER | LYS | SER | THR | TRP | LEU | ILE | LEU | HIS | ||||
| 4 | TYR | LYS | VAL | TYR | ASP | LEU | THR | LYS | PHE | LEU | ||||
| 5 | GLU | GLU | HIS | PRO | GLY | GLY | GLU | GLU | VAL | LEU | ||||
| 6 | ARG | GLU | GLN | ALA | GLY | GLY | ASP | ALA | THR | GLU | ||||
| 7 | ASN | PHE | GLU | ASP | VAL | GLY | HIS | SER | THR | ASP | ||||
| 8 | ALA | ARG | GLU | LEU | SER | LYS | THR | PHE | ILE | ILE | ||||
| 9 | GLY | GLU | LEU | HIS | PRO | ASP | ASP | ARG | SER | LYS | ||||
| 10 | ILE | THR | LYS | PRO | SER | GLU | SER | ILE | ILE | THR | ||||
| 11 | THR | ILE | ASP | SER |
Entity 2, HEM - C34 H32 Fe N4 O4 - 616.487 Da.
| 1 | HEM |
Samples:
sample_1: b5, [U-15N], 2.4 mM; phosphate 20 mM; HEM 2.4 mM; D2O 100%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 301 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
FELIX v2.3, Accelrys Software Inc. - processing
NMR spectrometers:
- Bruker AMX 600 MHz
- Varian UnityPlus 500 MHz