BMRB Entry 4970

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4970

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Title:
NMR Relaxation data for Protein Calmodulin in complex with the smooth muscle myosin light chain kinase calmodulin binding domain
Deposition date:
2001-03-12
Original release date:
2003-06-09
Authors:
Lee, Andrew; Wand, A.
Citation:

Citation: Lee, Andrew; Wand, A.. "NMR Relaxation data for Protein Calmodulin in complex with the smooth muscle myosin light chain kinase calmodulin binding domain"  Nature 411, 501-504 (2001).
PubMed: 11373686

Assembly members:

Assembly members:
Calmodulin, polymer, 148 residues, Formula weight is not available
smooth muscle myosin light chain kinase calmodulin binding domain, polymer, 21 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGD GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
smooth muscle myosin light chain kinase calmodulin binding domain: GSARRKWQKTGHAVRAIGRL S

Data sets:
Data typeCount
T1 relaxation values442
T2 relaxation values2320
order parameters1143

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin1
2smooth muscle myosin light chain kinase2

Entities:

Entity 1, Calmodulin 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASP
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, smooth muscle myosin light chain kinase 21 residues - Formula weight is not available

1   GLYSERALAARGARGLYSTRPGLNLYSTHR
2   GLYHISALAVALARGALAILEGLYARGLEU
3   SER

Samples:

sample_one: Calmodulin, [U-13C; U-15N], 1.2 mM; smooth muscle myosin light chain kinase calmodulin binding domain 2.4 mM; imidazole, [U-2H], 10 – 20 mM; KCl 100 mM; CaCl2 6 mM; NaN3 0.02%; H2O 93%; D2O 7%

sample_two: Calmodulin, [U-50% 2H; U-13C; U-15N], 1.2 mM; smooth muscle myosin light chain kinase calmodulin binding domain 2.4 mM; bis-tris, [U-2H], 10 – 20 mM; KCl 100 mM; CaCl2 6 mM; NaN3 0.02%; H2O 93%; D2O 7%

sample_conditions_1: pH: 5.0; temperature: 295 K

sample_conditions_2: pH: 5.0; temperature: 308 K

sample_conditions_3: pH: 5.0; temperature: 320 K

sample_conditions_4: pH: 5.0; temperature: 333 K

sample_conditions_5: pH: 5.0; temperature: 346 K

sample_conditions_6: pH: 5.0; temperature: 288 K

sample_conditions_7: pH: 5.0; temperature: 301 K

sample_conditions_8: pH: 5.0; temperature: 311 K

sample_conditions_9: pH: 5.0; temperature: 314 K

sample_conditions_10: pH: 5.0; temperature: 317 K

sample_conditions_11: pH: 5.0; temperature: 323 K

sample_conditions_12: pH: 5.0; temperature: 326 K

sample_conditions_13: pH: 5.0; temperature: 340 K

Experiments:

NameSampleSample stateSample conditions
15N T1not availablenot availablenot available
15N T2not availablenot availablenot available
15N T1rhonot availablenot availablenot available
{1H}-15N NOEnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 750 MHz

Related Database Links:

SWISS-PROT P62149 P62151 P21251 P62144 P02594 P11799
REF NP_001084025 NP_001039714 NP_001080864 NP_001008160 NP_001009759 NP_990790 NP_001099152
PIR JC1305 MCON
GenBank AAA40862 AAA40863 AAA35641 AAA37365 AAA35635 AAC29031 AAB41402 AAB53767 AAA49069 AAA69964
EMBL CAA32478 CAA32119 CAA32120 CAA32050 CAA32062 CAA37059 CAF90187 CAA37056 CAA37057
DBJ BAB28116 BAB28319 BAA11896 BAB23462 BAA08302 BAF80632
PDB
BMRB 6023 6541 5480 5770 5286 5287 4310 5227 4270 4284 15191 4056 15187 15188 15185 15186 15183 15184 5227 15184 15186