BMRB Entry 6699

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6699
MolProbity Validation Chart

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Title:
Backbone 1H, 13C, 15N and heavy atoms Chemical Shift Assignments for La3+ monosubstituted calbindin D9k
Deposition date:
2005-06-22
Original release date:
2006-04-27
Authors:
Balayssac, Stephane; Jimenez, Beatriz; Piccioli, Mario
Citation:

Citation: Balayssac, Stephane; Jimenez, Beatriz; Piccioli, Mario. "Assignment Strategy for Fast Relaxing Signals: Complete Aminoacid Identification in Thulium Substituted Calbindin D(9K)"  J. Biomol. NMR 34, 63-73 (2006).
PubMed: 16518694

Assembly members:

Assembly members:
Calcium Protein, polymer, 75 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
LA, non-polymer, 138.905 Da.

Natural source:

Natural source:   Common Name: Cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Calcium Protein: KSPEELKGIFEKYAAKEGDP NQLSKEELKLLLQTEFPSLL KGMSTLDELFEELDKNGDGE VSFEEFQVLVKKISQ

Data sets:
Data typeCount
13C chemical shifts256
15N chemical shifts77
1H chemical shifts159

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Calbindin D9k1
2CALCIUM (II) ION2
3LANTHANUM (III) ION3

Entities:

Entity 1, Calbindin D9k 75 residues - Formula weight is not available

1   LYSSERPROGLUGLULEULYSGLYILEPHE
2   GLULYSTYRALAALALYSGLUGLYASPPRO
3   ASNGLNLEUSERLYSGLUGLULEULYSLEU
4   LEULEUGLNTHRGLUPHEPROSERLEULEU
5   LYSGLYMETSERTHRLEUASPGLULEUPHE
6   GLUGLULEUASPLYSASNGLYASPGLYGLU
7   VALSERPHEGLUGLUPHEGLNVALLEUVAL
8   LYSLYSILESERGLN

Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.

1   CA

Entity 3, LANTHANUM (III) ION - La - 138.905 Da.

1   LA

Samples:

sample_1: Calbindin D9k, [U-95% 13C; U-98% 15N], 1 mM; CALCIUM (II) ION 1 mM; LANTHANUM (III) ION 1 mM

conditions_1: pH: 6.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H,15N-HSQCsample_1not availableconditions_1
1H,13C-HSQCsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
1D 13Csample_1not availableconditions_1
13C-13C COSYsample_1not availableconditions_1
CACOsample_1not availableconditions_1
CBCACOsample_1not availableconditions_1
CONsample_1not availableconditions_1
1D 15Nsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 500 MHz
  • Bruker Avance 400 MHz

Related Database Links:

PDB
BMRB 15594 16340 16758 247 325 326 327 4581 5207 6697 753 939 940
GB AAA30420 AAA72542 AAI18481 ADO85633
PRF 0707237A:PDB=3ICB
REF NP_776682 XP_004021986 XP_005701114 XP_005888771 XP_005982038
SP P02633
TPG DAA12577
AlphaFold P02633

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks