BMRB Entry 16340

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16340

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
100% complete assignment of non-labile 1H, 13C, and 15N signals for calcium-loaded Calbindin D9K P43G
Deposition date:
2009-06-09
Original release date:
2009-06-11
Authors:
Oktaviani, Nur Alia; Otten, Renee; Dijkstra, Klaas; Scheek, Ruud; Thulin, Eva; Akke, Mikael; Mulder, Frans
Citation:

Citation: Oktaviani, Nur Alia; Otten, Renee; Dijkstra, Klaas; Scheek, Ruud; Thulin, Eva; Akke, Mikael; Mulder, Frans. "100% complete assignment of non-labile (1)H, (13)C, and (15)N signals for calcium-loaded calbindin D (9k) P43G."  Biomol. NMR Assignments 5, 79-84 (2011).
PubMed: 21069485

Assembly members:

Assembly members:
Calbindin_D9K, polymer, 76 residues, 8591.7 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PCBWR

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Calbindin_D9K: MKSPEELKGIFEKYAAKEGD PNQLSKEELKLLLQTEFPSL LKGGSTLDELFEELDKNGDG EVSFEEFQVLVKKISQ

Data sets:
Data typeCount
13C chemical shifts386
15N chemical shifts92
1H chemical shifts565

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calbindin D9K1
2CA2

Entities:

Entity 1, Calbindin D9K 76 residues - 8591.7 Da.

1   METLYSSERPROGLUGLULEULYSGLYILE
2   PHEGLULYSTYRALAALALYSGLUGLYASP
3   PROASNGLNLEUSERLYSGLUGLULEULYS
4   LEULEULEUGLNTHRGLUPHEPROSERLEU
5   LEULYSGLYGLYSERTHRLEUASPGLULEU
6   PHEGLUGLULEUASPLYSASNGLYASPGLY
7   GLUVALSERPHEGLUGLUPHEGLNVALLEU
8   VALLYSLYSILESERGLN

Entity 2, CA - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Calbindin D9K, [U-95% 13C; U-95% 15N], 2.5 mM; DSS 0.1 mM; sodium azide 0.01%; Cyclic Enterobacterial Common Antigen, [U-95% 13C; U-95% 15N], 0 – 1 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0 M; pH: 6; pressure: 1 atm; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D HA(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)Nsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
2D H(N)COsample_1isotropicsample_conditions_1
2D HACA(N)sample_1isotropicsample_conditions_1
2D H2(CA)Nsample_1isotropicsample_conditions_1
3D HCCH COSYsample_1isotropicsample_conditions_1
3D 15N-1H-TOCSY-HSQCsample_1isotropicsample_conditions_1
3D H(CCO)NH-TOCSYsample_1isotropicsample_conditions_1
3D (H)C(CO)NH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D H2(CA)Nsample_1isotropicsample_conditions_1
2D 3JNCgammasample_1isotropicsample_conditions_1
2D 3JCOCgammasample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D CG(CB)HBsample_1isotropicsample_conditions_1
2D CB(CGCD)HDsample_1isotropicsample_conditions_1
2D CB(CGCDCE)HEsample_1isotropicsample_conditions_1
3D HCCH-COSY Aromaticsample_1isotropicsample_conditions_1
2D 13C-1H HSQC Aromaticsample_1isotropicsample_conditions_1
2D 13C-1H HSQC Aromatic Cross Polarizationsample_1isotropicsample_conditions_1
2D 13C-1H HMQC Aromaticsample_1isotropicsample_conditions_1
2D H(C)COsample_1isotropicsample_conditions_1
2D (HBGCBG)CO(CBGCABCACON)Hsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

GB AAA72542.1 AAA30420 AAA72542 AAI18481 ADO85633
SWS P02633
BMRB 15594 16758 247 325 326 327 4581
PDB
PRF 0707237A:PDB=3ICB
REF NP_776682 XP_004021986 XP_005701114 XP_005888771 XP_005982038
SP P02633
TPG DAA12577
AlphaFold P02633

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks