BMRB Entry 5207
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5207
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Nelson, Melanie; Thulin, Eva; Fagan, Patricia; Forsen, Sture; Chazin, Walter. "The EF-hand Domain: A Globally Cooperative Structural unit" Protein Sci. 11, 198-205 (2002).
PubMed: 11790829
Assembly members:
CALBINDIN D9K, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: Bovine Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRCB1
Entity Sequences (FASTA):
CALBINDIN D9K: MKSPEELKGIFEKYAAKEGD
PNQLSKEELKLLLQTEGPSL
LKGMSTLDELFEELDKNGDG
EVSFEEFQVLVKKISQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 518 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CALBINDIN D9K F36G | 1 |
Entities:
Entity 1, CALBINDIN D9K F36G 76 residues - Formula weight is not available
| 1 | MET | LYS | SER | PRO | GLU | GLU | LEU | LYS | GLY | ILE | ||||
| 2 | PHE | GLU | LYS | TYR | ALA | ALA | LYS | GLU | GLY | ASP | ||||
| 3 | PRO | ASN | GLN | LEU | SER | LYS | GLU | GLU | LEU | LYS | ||||
| 4 | LEU | LEU | LEU | GLN | THR | GLU | GLY | PRO | SER | LEU | ||||
| 5 | LEU | LYS | GLY | MET | SER | THR | LEU | ASP | GLU | LEU | ||||
| 6 | PHE | GLU | GLU | LEU | ASP | LYS | ASN | GLY | ASP | GLY | ||||
| 7 | GLU | VAL | SER | PHE | GLU | GLU | PHE | GLN | VAL | LEU | ||||
| 8 | VAL | LYS | LYS | ILE | SER | GLN |
Samples:
sample_1: CALBINDIN D9K 2.5 mM; H2O 100%
sample_2: CALBINDIN D9K 2.5 mM; D2O 100%
sample_3: CALBINDIN D9K, [U-15N], 2.5 mM
sample_cond_1: pH: 6.0; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D COSY | not available | not available | sample_cond_1 |
| 2D NOESY | not available | not available | sample_cond_1 |
| 2D TOCSY | not available | not available | sample_cond_1 |
| 2D 15N-1H HSQC | not available | not available | sample_cond_1 |
| 3D 15N-separated NOESY | not available | not available | sample_cond_1 |
| 3D 15N-separated TOCSY | not available | not available | sample_cond_1 |
| 3D HNHA | not available | not available | sample_cond_1 |
| 3D HNHB | not available | not available | sample_cond_1 |
Software:
FELIX v97.0 - data processing, resonance assignments
DIANA v2.8 - calculation of starting structures
AMBER v4.1 - structure refinement
GENXPK v1 - resonance and NOE assignments
GLOMSA - determine dihedral angle constraints
NMR spectrometers:
- BRUKER DMX 750 MHz
- BRUKER AMX 500 MHz