BMRB Entry 4581

Name: Site-site Communication in the EF-hand Ca2+ Binding Protein Calbindin D9k
Published: 2000-12-18

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PDB ID: 1d1o
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4581
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Site-site Communication in the EF-hand Ca2+ Binding Protein Calbindin D9k

Deposition date:

2000-02-02

Original release date:

2000-12-18

Authors:

Maler, L.; Blankenship, J.; Rance, M.; Chazin, W.

Citation:

Citation: Maler, L.; Blankenship, J.; Rance, M.; Chazin, W.. "Site-site Communication in the EF-hand Ca2+ Binding Protein Calbindin D9k" Nat. Struct. Biol. 7, 245-250 (2000).

Assembly members:

Assembly members:
CALBINDIN D9K, polymer, 75 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bovine Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CALBINDIN D9K: KSPEELKGIFEKYAAKEGDP NQLSKEELKLLLQTEFPSLL KGMSTLDELFEELDKAGDGE VSFEEFQVLVKKISQ

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	480
15N chemical shifts	70

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CALBINDIN D9K	1

Entities:

Entity 1, CALBINDIN D9K 75 residues - Formula weight is not available

1

LYS

SER

PRO

GLU

LEU

LYS

GLY

ILE

PHE

2

GLU

LYS

TYR

ALA

LYS

GLU

GLY

ASP

PRO

3

ASN

GLN

LEU

SER

LYS

GLU

LEU

LYS

LEU

4

LEU

GLN

THR

GLU

PHE

PRO

SER

LEU

5

LYS

GLY

MET

SER

THR

LEU

ASP

GLU

LEU

PHE

6

GLU

LEU

ASP

LYS

ALA

GLY

ASP

GLY

GLU

7

VAL

SER

PHE

GLU

PHE

GLN

VAL

LEU

VAL

8

LYS

ILE

SER

GLN

Samples:

sample_1: CALBINDIN D9K 4 mM

sample_2: CALBINDIN D9K, [U-15N], 4 mM

sample_cond_1: pH: 6.0; temperature: 300 K; ionic strength: 0 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	sample_cond_1
3D 15N-SEPARATED NOESY	not available	not available	sample_cond_1
HSQC-J	not available	not available	sample_cond_1

Software:

FELIX v97.0 - resonance assignments, bookkeeping

DIANA v2.8 - structure solution

AMBER v4.1 - refinement

NMR spectrometers:

Bruker AMX 500 MHz
Bruker DRX 600 MHz
Varian Inova 600 MHz

BMRB	15594 16340 16758 247 325 326 327
PDB	1B1G 1CDN 1CLB 1D1O 1HT9 1IG5 1IGV 1KCY 1KQV 1KSM 1N65 2BCA 2BCB 2MAZ 3ICB 4ICB
GB	AAA30420 AAA72542 AAI18481
PRF	0707237A:PDB=3ICB
REF	NP_776682 XP_004021986 XP_005701114 XP_005888771 XP_005982038
SP	P02633
TPG	DAA12577
AlphaFold	P02633