BMRB Entry 16758

Title:
The effect of protein concentration on ion binding
Deposition date:
2010-03-03
Original release date:
2010-03-24
Authors:
Linse, Sara; Jonsson, Bo; Chazin, Walter
Citation:

Citation: Linse, Sara; Jonsson, Bo; Chazin, Walter. "The effect of protein concentration on ion binding"  Proc. Natl. Acad. Sci. U.S.A. 92, 4748-4752 (1995).
PubMed: 7761395

Assembly members:

Assembly members:
calbindin, polymer, 75 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: cattle   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: na

Entity Sequences (FASTA):

Data sets:
  • binding_constants
Data typeCount
binding constants13

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calbindin1
2CA2

Entities:

Entity 1, calbindin 75 residues - Formula weight is not available

1   LYSSERPROGLUGLULEULYSGLYILEPHE
2   GLULYSTYRALAALALYSGLUGLYASPPRO
3   ASNGLNLEUSERLYSGLUGLULEULYSLEU
4   LEULEUGLNTHRGLUPHEPROSERLEULEU
5   LYSGLYPROSERTHRLEUASPGLULEUPHE
6   GLUGLULEUASPLYSALAGLYASPGLYGLU
7   VALSERPHEGLUGLUPHEGLNVALLEUVAL
8   LYSLYSILESERGLN

Entity 2, CA - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Tris-HCl 2 mM; calbindin D9k(N56A)0.027 – 3.30 mM; CaCl2 0.027 mM; sodium0.14 – 17.2 mM; potassium chloride0 – 1000 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1H NMRsample_1isotropicsample_conditions_1

Software:

not_given, not given - not given

NMR spectrometers:

  • GE OMEGA 500 MHz

Related Database Links:

BMRB 15594 16340 247 325 326 327 4581
PDB
GB AAA30420 AAA72542 AAI18481 ADO85633
PRF 0707237A:PDB=3ICB
REF NP_776682 XP_004021986 XP_005701114 XP_005888771 XP_005982038
SP P02633
TPG DAA12577
AlphaFold P02633