BMRB Entry 16758
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16758
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Citation: Linse, Sara; Jonsson, Bo; Chazin, Walter. "The effect of protein concentration on ion binding" Proc. Natl. Acad. Sci. U.S.A. 92, 4748-4752 (1995).
PubMed: 7761395
Assembly members:
calbindin, polymer, 75 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
Natural source: Common Name: cattle Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: na
Entity Sequences (FASTA):
calbindin: KSPEELKGIFEKYAAKEGDP
NQLSKEELKLLLQTEFPSLL
KGPSTLDELFEELDKAGDGE
VSFEEFQVLVKKISQ
- binding_constants
| Data type | Count |
| binding constants | 13 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | calbindin | 1 |
| 2 | CA | 2 |
Entities:
Entity 1, calbindin 75 residues - Formula weight is not available
| 1 | LYS | SER | PRO | GLU | GLU | LEU | LYS | GLY | ILE | PHE | ||||
| 2 | GLU | LYS | TYR | ALA | ALA | LYS | GLU | GLY | ASP | PRO | ||||
| 3 | ASN | GLN | LEU | SER | LYS | GLU | GLU | LEU | LYS | LEU | ||||
| 4 | LEU | LEU | GLN | THR | GLU | PHE | PRO | SER | LEU | LEU | ||||
| 5 | LYS | GLY | PRO | SER | THR | LEU | ASP | GLU | LEU | PHE | ||||
| 6 | GLU | GLU | LEU | ASP | LYS | ALA | GLY | ASP | GLY | GLU | ||||
| 7 | VAL | SER | PHE | GLU | GLU | PHE | GLN | VAL | LEU | VAL | ||||
| 8 | LYS | LYS | ILE | SER | GLN |
Entity 2, CA - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: Tris-HCl 2 mM; calbindin D9k(N56A)0.027 – 3.30 mM; CaCl2 0.027 mM; sodium0.14 – 17.2 mM; potassium chloride0 – 1000 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H NMR | sample_1 | isotropic | sample_conditions_1 |
Software:
not_given, not given - not given
NMR spectrometers:
- GE OMEGA 500 MHz