BMRB Entry 4808

Title:
1H, 13C and 15N chemical shifts of bovine ferrous cytochrome b5 in complex with horse heart ferrous cytochrome c
Deposition date:
2000-08-14
Original release date:
2000-11-15
Authors:
Hom, Kellie; Ma, Qi-Feng; Wolfe, Gary; Zhang, Hong; Storch, Elizabeth; Daggett, Valerie; Basus, Vladimir; Waskell, Lucy
Citation:

Citation: Hom, Kellie; Ma, Qi-Feng; Wolfe, Gary; Zhang, Hong; Storch, Elizabeth; Daggett, Valerie; Basus, Vladimir; Waskell, Lucy. "NMR Studies of the Association of Cytochrome b5 with Cytochrome c"  Biochemistry ., .-..

Assembly members:

Assembly members:
ferrous cytochrome b5, polymer, 82 residues, 9966 Da.
cytochrome c, polymer, 104 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pUC19

Data sets:
Data typeCount
1H chemical shifts484
13C chemical shifts212
15N chemical shifts78

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytb51
2cytc2
3heme3
4heme c4

Entities:

Entity 1, cytb5 82 residues - 9966 Da.

1   ALAVALLYSTYRTYRTHRLEUGLUGLUILE
2   GLNLYSHISASNASPSERLYSSERTHRTRP
3   LEUILELEUHISTYRLYSVALTYRASPLEU
4   THRLYSPHELEUGLUGLUHISPROGLYGLY
5   GLUGLUVALLEUARGGLUGLNALAGLYGLY
6   ASPALATHRGLUASNPHEGLUASPVALGLY
7   HISSERTHRASPALAARGGLULEUSERLYS
8   THRPHEILEILEGLYGLULEUHISPROASP
9   ASPARG

Entity 2, cytc 104 residues - Formula weight is not available

1   GLYASPVALGLULYSGLYLYSLYSILEPHE
2   VALGLNLYSCYSALAGLNCYSHISTHRVAL
3   GLULYSGLYGLYLYSHISLYSTHRGLYPRO
4   ASNLEUHISGLYLEUPHEGLYARGLYSTHR
5   GLYGLNALAPROGLYPHETHRTYRTHRASP
6   ALAASNLYSASNLYSGLYILETHRTRPLYS
7   GLUGLUTHRLEUMETGLUTYRLEUGLUASN
8   PROLYSLYSTYRILEPROGLYTHRLYSMET
9   ILEPHEALAGLYILELYSLYSLYSTHRGLU
10   ARGGLUASPLEUILEALATYRLEULYSLYS
11   ALATHRASNGLU

Entity 3, heme - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Entity 4, heme c - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: ferrous cytochrome b5, [U-90% 13C; U-90% 15N], 0.3 mM; PROTOPORPHYRIN IX CONTAINING FE mM; cytochrome c mM

ex-cond_1: pH*: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-1H HSQCsample_1not availableex-cond_1
13C-1H HSQCsample_1not availableex-cond_1
HNCAsample_1not availableex-cond_1
HCCH-TOCSYsample_1not availableex-cond_1
2D version of ct-HCACOsample_1not availableex-cond_1
2D version of HNCOCAsample_1not availableex-cond_1
mq-HNCOsample_1not availableex-cond_1

Software:

Striker - FID transformation

nmrPipe - FID transformation

Sparky v3.3.2 - Spectral analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian Unity plus 600 MHz

Related Database Links:

BMRB 1058 1107 1108 1109 1110 1111 1112 1113 1114 1116 1170 1171 1404 16759 17120 17215 17340 1736 1783 17847 17848 1785 1787 1789 216 220 224 2366 2367 2368 243 244 274 285 286 316 317 336 4189 436 437 438 439 4805 4809 4810 499 5026 5372 543 544 545 546 5660 5827 5828 5829 5830 630 645 649 665 672 673 944 946 947 948 948 947 946 944 673 672 665 645 630 5830 5829 5828 5827 5660 546 545 544 543 5372 5026 499 4810 4809 4805 439 438 437 436 4189 336 317 316 286 285 274 244 243 2368 2367 2366 224 220 216 1789 1787 1785 1783 1736 1404 1171 1170 1116 1114 1113 1112 1111 1110 1109 1108 1107 1058
PDB
GB AAB33495 AAI05398 AAX77008 ABA06541 AEB61027
PRF 610169A 711086A 610169A
REF NP_001039526 NP_001123442 NP_001157486 XP_004007999 XP_004418964 XP_001498872
SP P00004 P62894 P62895 P62896 P68096
TPG DAA30512
SWISS-PROT P68097 P68096 P00004
GenBank AAB33495
AlphaFold P62896 P00004 P62894 P68096 P62895

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks