BMRB Entry 4804
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4804
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Citation: Hom, Kellie; Ma, Qi-Feng; Wolfe, Gary; Zhang, Hong; Storch, Elizabeth; Daggett, Valerie; Basus, Vladimir; Waskell, Lucy. "NMR Studies of the Association of Cytochrome b5 with Cytochrome c" Biochemistry ., .-..
Assembly members:
ferrous cytochrome b5, polymer, 82 residues, 9966 Da.
HEM, non-polymer, Formula weight is not available
Natural source: Common Name: bovine Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pUC19
Entity Sequences (FASTA):
ferrous cytochrome b5: AVKYYTLEEIQKHNDSKSTW
LILHYKVYDLTKFLEEHPGG
EEVLREQAGGDATENFEDVG
HSTDARELSKTFIIGELHPD
DR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 578 |
| 13C chemical shifts | 373 |
| 15N chemical shifts | 88 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cytb5-N17D | 1 |
| 2 | heme | 2 |
Entities:
Entity 1, cytb5-N17D 82 residues - 9966 Da.
| 1 | ALA | VAL | LYS | TYR | TYR | THR | LEU | GLU | GLU | ILE | ||||
| 2 | GLN | LYS | HIS | ASN | ASP | SER | LYS | SER | THR | TRP | ||||
| 3 | LEU | ILE | LEU | HIS | TYR | LYS | VAL | TYR | ASP | LEU | ||||
| 4 | THR | LYS | PHE | LEU | GLU | GLU | HIS | PRO | GLY | GLY | ||||
| 5 | GLU | GLU | VAL | LEU | ARG | GLU | GLN | ALA | GLY | GLY | ||||
| 6 | ASP | ALA | THR | GLU | ASN | PHE | GLU | ASP | VAL | GLY | ||||
| 7 | HIS | SER | THR | ASP | ALA | ARG | GLU | LEU | SER | LYS | ||||
| 8 | THR | PHE | ILE | ILE | GLY | GLU | LEU | HIS | PRO | ASP | ||||
| 9 | ASP | ARG |
Entity 2, heme - C34 H32 N4 O4 FE1 - Formula weight is not available
| 1 | HEM |
Samples:
sample_1: ferrous cytochrome b5, [U-90% 13C; U-90% 15N], 2 – 9 mM; PROTOPORPHYRIN IX CONTAINING FE2 – 9 mM
ex-cond_1: pH*: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | not available | ex-cond_1 |
| 13C-1H HSQC | sample_1 | not available | ex-cond_1 |
| (HB)CB(CGCD)HD | sample_1 | not available | ex-cond_1 |
| (HB)CB(CGCDCE)HE | sample_1 | not available | ex-cond_1 |
| 1H-TOCSY-relayed ct-[13C, 1H]-HMQC | sample_1 | not available | ex-cond_1 |
| HNCO | sample_1 | not available | ex-cond_1 |
| HNCA | sample_1 | not available | ex-cond_1 |
| HNCACB | sample_1 | not available | ex-cond_1 |
| CBCA(CO)NNH | sample_1 | not available | ex-cond_1 |
| HBHA(CO)NNH | sample_1 | not available | ex-cond_1 |
| HCCH-TOCSY | sample_1 | not available | ex-cond_1 |
| ct-HCACO | sample_1 | not available | ex-cond_1 |
| 13C-edited NOESY-HMQC | sample_1 | not available | ex-cond_1 |
| 15N-edited NOESY-HMQC | sample_1 | not available | ex-cond_1 |
Software:
Striker - FID transformation
nmrPipe - FID transformation
Sparky v3.3.2 - Spectral analysis
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian Unity plus 600 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks