BMRB Entry 5728
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5728
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Citation: Assadi-Porter, Fariba; Abilgaard, Frits; Blad, Heike; Markley, John. "Correlation of the Sweetness of Variants of the Protein Brazzein with
Patterns of Hydrogen Bonds Detected by NMR Spectroscopy" J. Biol. Chem. 278, 31331-31339 (2003).
PubMed: 12732626
Assembly members:
brazzein, polymer, 53 residues, Formula weight is not available
Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
brazzein: DKCKKVYENYPVSKCQLANQ
CNYDCKLDKHAASGECFYDE
KRNLQCICDYCEY
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 49 |
| 15N chemical shifts | 50 |
| 1H chemical shifts | 50 |
| coupling constants | 14 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Arg33Ala-brazzein | 1 |
Entities:
Entity 1, Arg33Ala-brazzein 53 residues - Formula weight is not available
| 1 | ASP | LYS | CYS | LYS | LYS | VAL | TYR | GLU | ASN | TYR | ||||
| 2 | PRO | VAL | SER | LYS | CYS | GLN | LEU | ALA | ASN | GLN | ||||
| 3 | CYS | ASN | TYR | ASP | CYS | LYS | LEU | ASP | LYS | HIS | ||||
| 4 | ALA | ALA | SER | GLY | GLU | CYS | PHE | TYR | ASP | GLU | ||||
| 5 | LYS | ARG | ASN | LEU | GLN | CYS | ILE | CYS | ASP | TYR | ||||
| 6 | CYS | GLU | TYR |
Samples:
sample_1: brazzein, [U-100% 13C; U-100% 15N], 2.0 – 4.0 mM; 2,2-dimethyl-2-silapentane-5-sulfonate 0.1 mM; sodium azide 1 mM; succinic acid, [U-2H], 2 mM
sample_2: brazzein, [U-100% 15N], 2.0 mM
condition_1: ionic strength: 2 mM; pH: 5.2 na; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCOCA | not available | not available | not available |
| HNCA | not available | not available | not available |
| 1H-1H-15N TOCSY | not available | not available | not available |
| 1H-1H-15N NOESY | not available | not available | not available |
| long range HNCO | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 600 MHz
- Bruker DMX 750 MHz
Related Database Links:
| BMRB | 16215 18710 4067 5295 5723 5725 |
| PDB | |
| GB | ACK76425 AGM38242 |
| SP | P56552 |
| AlphaFold | P56552 |
Download HSQC peak lists in one of the following formats:
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