BMRB Entry 4067
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4067
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Citation: Caldwell, Jane; Abildgaard, Frits; Ming, Ding; Hellekant, Goran; Markley, John. "Complete 1H and Partial 13C Resonance Assignments at 37 and 22 C
for Brazzein, an Intensely Sweet Protein" J. Biomol. NMR 11, 231-232 (1998).
Assembly members:
brazzein, polymer, 54 residues, 6474 Da.
Natural source: Common Name: Joublie fruit Taxonomy ID: 43545 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Pentadiplandra brazzeana
Experimental source: Production method: purified from natural source
Entity Sequences (FASTA):
brazzein: XDKCKKVYENYPVSKCQLAN
QCNYDCKLDKHARSGECFYD
EKRNLQCICDYCEY
| Data type | Count |
| 13C chemical shifts | 126 |
| 1H chemical shifts | 1019 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | brazzein | 1 |
Entities:
Entity 1, brazzein 54 residues - 6474 Da.
| 1 | PCA | ASP | LYS | CYS | LYS | LYS | VAL | TYR | GLU | ASN | ||||
| 2 | TYR | PRO | VAL | SER | LYS | CYS | GLN | LEU | ALA | ASN | ||||
| 3 | GLN | CYS | ASN | TYR | ASP | CYS | LYS | LEU | ASP | LYS | ||||
| 4 | HIS | ALA | ARG | SER | GLY | GLU | CYS | PHE | TYR | ASP | ||||
| 5 | GLU | LYS | ARG | ASN | LEU | GLN | CYS | ILE | CYS | ASP | ||||
| 6 | TYR | CYS | GLU | TYR |
Samples:
sample_1: brazzein0.8 – 2 mM; sodium azide 1 mM; DSS 0.1 mM
sample_2: brazzein1 – 4 mM; succinic-d4 acid, [U-99% 2H], 50 mM; DSS 0.1 mM
sample_3: brazzein 8 mM; succinic-d4 acid, [U-99% 2H], 50 mM; DSS 0.1 mM; sodium azide 1 mM
conditions_set_1: pH: 3.7; temperature: 310 K
conditions_set_2: pH: 5.2; temperature: 310 K
conditions_set_3: pH: 5.2; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Gradient enhanced 1H,13C HSQC | sample_2 | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker AM600 600 MHz
- Bruker DMX600 600 MHz
- Bruker DMX750 750 MHz