BMRB Entry 5725
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5725
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Citation: Assadi-Porter, Fariba; Abilgaard, Frits; Blad, Heike; Markley, John. "Correlation of the Sweetness of Variants of the Protein Brazzein with
Patterns of Hydrogen Bonds Detected by NMR Spectroscopy" J. Biol. Chem. 278, 31331-31339 (2003).
PubMed: 12732626
Assembly members:
brazzein, polymer, 54 residues, Formula weight is not available
Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
brazzein: ADKCKKVYENYPVSKCQLAN
QCNYDCKLDKHARSGECFYD
EKRNLQCICDYCEY
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 50 |
| 15N chemical shifts | 50 |
| 1H chemical shifts | 50 |
| coupling constants | 17 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ala2insertion-brazzein | 1 |
Entities:
Entity 1, Ala2insertion-brazzein 54 residues - Formula weight is not available
| 1 | ALA | ASP | LYS | CYS | LYS | LYS | VAL | TYR | GLU | ASN | ||||
| 2 | TYR | PRO | VAL | SER | LYS | CYS | GLN | LEU | ALA | ASN | ||||
| 3 | GLN | CYS | ASN | TYR | ASP | CYS | LYS | LEU | ASP | LYS | ||||
| 4 | HIS | ALA | ARG | SER | GLY | GLU | CYS | PHE | TYR | ASP | ||||
| 5 | GLU | LYS | ARG | ASN | LEU | GLN | CYS | ILE | CYS | ASP | ||||
| 6 | TYR | CYS | GLU | TYR |
Samples:
sample_1: brazzein, [U-100% 13C; U-100% 15N], 2.0 – 4.0 mM; 2,2-dimethyl-2-silapentane-5-sulfonate 0.1 mM; sodium azide 1 mM
sample_2: brazzein, [U-100% 15N], 2.0 mM
condition_1: ionic strength: 2 mM; pH: 5.2 na; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| 1H-1H-15N TOCSY | not available | not available | not available |
| 1H-1H-15N NOESY | not available | not available | not available |
| long range HNCO (H-bond) | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 600 MHz
- Bruker DMX 750 MHz
Related Database Links:
| BMRB | 16215 16978 18710 4067 5295 5723 5724 5726 5727 5728 |
| PDB | |
| GB | ACK76425 AGM38242 |
| SP | P56552 |
| AlphaFold | P56552 |
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SPARKY: Backbone
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