BMRB Entry 18710

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PDB ID: 2ly6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18710
MolProbity Validation Chart

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Title:
Refined solution structure of recombinant brazzein at low temperature
Deposition date:
2012-09-12
Original release date:
2012-10-15
Authors:
Cornilescu, Claudia; Cornilescu, Gabriel; Tonelli, Marco; Markley, John; Assadi-Porter, Fariba
Citation:

Citation: Cornilescu, Claudia; Cornilescu, Gabriel; Rao, Hongyu; Porter, Sarah; Tonelli, Marco; Derider, Michele; Markley, John; Assadi-Porter, Fariba. "Temperature-dependent conformational change affecting Tyr11 and sweetness loops of brazzein."  Proteins 81, 919-925 (2013).
PubMed: 23349025

Assembly members:

Assembly members:
entity, polymer, 53 residues, 6380.253 Da.

Natural source:

Natural source:   Common Name: Pentadiplandra brazzeana baillon   Taxonomy ID: 43545   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Pentadiplandra brazzeana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: DKCKKVYENYPVSKCQLANQ CNYDCKLDKHARSGECFYDE KRNLQCICDYCEY

Data sets:
Data typeCount
13C chemical shifts223
15N chemical shifts59
1H chemical shifts328

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
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Assembly:

Entity Assembly IDEntity NameEntity ID
1recombinant brazzein1

Entities:

Entity 1, recombinant brazzein 53 residues - 6380.253 Da.

1   ASPLYSCYSLYSLYSVALTYRGLUASNTYR
2   PROVALSERLYSCYSGLNLEUALAASNGLN
3   CYSASNTYRASPCYSLYSLEUASPLYSHIS
4   ALAARGSERGLYGLUCYSPHETYRASPGLU
5   LYSARGASNLEUGLNCYSILECYSASPTYR
6   CYSGLUTYR

Related Database Links:

BMRB 16215 16978 4067
PDB 1BRZ 2BRZ 2KGQ 2KYQ 2LY5 2LY6 4HE7
GB ACK76425 AGM38242
SP P56552
AlphaFold P56552

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