BMRB Entry 18795

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18795

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Title:
NMR assignments of Amylin in DMSO
Deposition date:
2012-10-20
Original release date:
2013-04-02
Authors:
Alexandrescu, Andrei
Citation:

Citation: Alexandrescu, Andrei. "Amide proton solvent protection in amylin fibrils probed by quenched hydrogen exchange NMR"  PLOS One 8, e56467-e56467 (2013).
PubMed: 23457571

Assembly members:

Assembly members:
Amylin, polymer, 37 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Purchased from rPeptide - proprietary vector

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Amylin: KCNTATCATQRLANFLVHSS NNFGAILSSTNVGSNTY

Data sets:
Data typeCount
15N chemical shifts35
1H chemical shifts136

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Amylin1

Entities:

Entity 1, Amylin 37 residues - Formula weight is not available

The naturally occurring human amylin hormone has an amidated C-terminus. This is an enzymatic post-translational modification. The sample used for NMR was recombinantely expressed in E. coli and has a free alpha-caroxylic acid at the C-terminus.

1   LYSCYSASNTHRALATHRCYSALATHRGLN
2   ARGLEUALAASNPHELEUVALHISSERSER
3   ASNASNPHEGLYALAILELEUSERSERTHR
4   ASNVALGLYSERASNTHRTYR

Samples:

sample_1: Amylin, [U-99% 15N], 0.5 mM; DMSO-d6, [U-2H], 95%; DCA-d2, [U-2H], 5%

sample_conditions_1: ionic strength: 0 mM; pH*: 3.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian-Agilent Technologies - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16104 16105 17394 20045
PDB
DBJ BAG73319
EMBL CAA33032 CAA37002 CAA39504 CAA48724 CAB57803
GB AAA35524 AAA35983 AAA51728 AAA52281 AAI11850
REF NP_000406 XP_001144800 XP_003265632 XP_003265633 XP_003828947
SP P10997
AlphaFold P10997

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks