BMRB Entry 17394

Name: Solution NMR structure of human amylin in SDS micelles at pH 7.3
Published: 2012-06-19

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PDB ID: 2l86
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17394
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of human amylin in SDS micelles at pH 7.3

Deposition date:

2011-01-04

Original release date:

2012-06-19

Authors:

Nanga, Ravi Prakash Reddy; Brender, Jeffrey; Vivekanandan, Subramanian; Ramamoorthy, Ayyalusamy

Citation:

Citation: Nanga, Ravi Prakash Reddy; Brender, Jeffrey; Vivekanandan, Subramanian; Ramamoorthy, Ayyalusamy. "Structure and membrane orientation of IAPP in its natively amidated form at physiological pH in a membrane environment." Biochim. Biophys. Acta 1808, 2337-2342 (2011).
PubMed: 21723249

Assembly members:

Assembly members:
entity, polymer, 37 residues, 3909.330 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: KCNTATCATQRLANFLVHSS NNFGAILSSTNVGSNTY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	228

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	amidated IAPP	1

Entities:

Entity 1, amidated IAPP 37 residues - 3909.330 Da.

1

LYS

CYS

ASN

THR

ALA

THR

CYS

ALA

THR

GLN

2

ARG

LEU

ALA

ASN

PHE

LEU

VAL

HIS

SER

3

ASN

PHE

GLY

ALA

ILE

LEU

SER

THR

4

ASN

VAL

GLY

SER

ASN

THR

TYR

Samples:

sample_1: amidated IAPP 2.5 mM; sodium phosphate 20 mM; sodium chloride 120 mM; D2O, [U-99% 2H], 10%; H2O 90%; SDS, [U-99% 2H], 200 mM

sample_conditions_1: ionic strength: 20 mM; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

Molmol, Koradi, Billeter and Wuthrich - data analysis, structure solution

ProcheckNMR, Laskowski and MacArthur - data analysis

NMR spectrometers:

Bruker Avance 900 MHz

BMRB	16104 16105 18795 20045
PDB	2G48 2KB8 2L86 3G7V 3G7W 3HGZ
DBJ	BAG73319
EMBL	CAA33032 CAA37002 CAA39504 CAA48724 CAB57803
GB	AAA35524 AAA35983 AAA51728 AAA52281 AAI11850
REF	NP_000406 XP_001144800 XP_003265632 XP_003265633 XP_003828947
SP	P10997
AlphaFold	P10997