BMRB Entry 20045
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20045
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Citation: NANGA, RAVI PRAKASH REDDY; BRENDER, JEFFREY; XU, JIADI; VEGLIA, GIANLUIGI; RAMAMOORTHY, AYYALUSAMY. "Structures of rat and human islet amyloid polypeptide IAPP(1-19) in micelles by NMR spectroscopy" Biochemistry 47, 12689-12697 (2008).
PubMed: 18989932
Assembly members:
IAPP, polymer, 19 residues, 2081.410 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
IAPP: KCNTATCATQRLANFLVHS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 104 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IAPP | 1 |
Entities:
Entity 1, IAPP 19 residues - 2081.410 Da.
| 1 | LYS | CYS | ASN | THR | ALA | THR | CYS | ALA | THR | GLN | ||||
| 2 | ARG | LEU | ALA | ASN | PHE | LEU | VAL | HIS | SER |
Samples:
sample_1: entity 1.625 mM; DPC, [U-100% 2H], 200 mM; sodium azide 0.02%; sodium phosphate 20 mM; sodium chloride 120 mM; D2O 10%; H2O 90%
sample_conditions_1: pH: 7.3; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment, chemical shift calculation, data analysis
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution
TALOS, Cornilescu, Delaglio and Bax - refinement
ProcheckNMR, Laskowski and MacArthur - data analysis
NMR spectrometers:
- Bruker Avance 900 MHz