BMRB Entry 18479

Name: HRas166*GDP backbone chemical shift assignments
Published: 2013-02-28

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18479

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

HRas166*GDP backbone chemical shift assignments

Deposition date:

2012-05-24

Original release date:

2013-02-28

Authors:

Kovrigin, Evgenii; O'Connor, Casey

Citation:

Citation: Kovrigin, Casey. "Assignments of backbone H, C and N resonances in H-Ras (1-166) complexed with GppNHp at physiological pH." Biomol. NMR Assignments 6, 91-93 (2012).
PubMed: 21814767

Assembly members:

Assembly members:
HRas166, polymer, 166 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HRas166: MTEYKLVVVGAGGVGKSALT IQLIQNHFVDEYDPTIEDSY RKQVVIDGETCLLDILDTAG QEEYSAMRDQYMRTGEGFLC VFAINNTKSFEDIHQYREQI KRVKDSDDVPMVLVGNKCDL AARTVESRQAQDLARSYGIP YIETSAKTRQGVEDAFYTLV REIRQH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	469
15N chemical shifts	161
1H chemical shifts	161

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HRas166	1

Entities:

Entity 1, HRas166 166 residues - Formula weight is not available

1

MET

THR

GLU

TYR

LYS

LEU

VAL

GLY

2

ALA

GLY

VAL

GLY

LYS

SER

ALA

LEU

THR

3

ILE

GLN

LEU

ILE

GLN

ASN

HIS

PHE

VAL

ASP

4

GLU

TYR

ASP

PRO

THR

ILE

GLU

ASP

SER

TYR

5

ARG

LYS

GLN

VAL

ILE

ASP

GLY

GLU

THR

6

CYS

LEU

ASP

ILE

LEU

ASP

THR

ALA

GLY

7

GLN

GLU

TYR

SER

ALA

MET

ARG

ASP

GLN

8

TYR

MET

ARG

THR

GLY

GLU

GLY

PHE

LEU

CYS

9

VAL

PHE

ALA

ILE

ASN

THR

LYS

SER

PHE

10

GLU

ASP

ILE

HIS

GLN

TYR

ARG

GLU

GLN

ILE

11

LYS

ARG

VAL

LYS

ASP

SER

ASP

VAL

PRO

12

MET

VAL

LEU

VAL

GLY

ASN

LYS

CYS

ASP

LEU

13

ALA

ARG

THR

VAL

GLU

SER

ARG

GLN

ALA

14

GLN

ASP

LEU

ALA

ARG

SER

TYR

GLY

ILE

PRO

15

TYR

ILE

GLU

THR

SER

ALA

LYS

THR

ARG

GLN

16

GLY

VAL

GLU

ASP

ALA

PHE

TYR

THR

LEU

VAL

17

ARG

GLU

ILE

ARG

GLN

HIS

Samples:

sample_1: HRas166, [U-99% 13C; U-99% 15N], 0.95 ± 0.1 mM; magnesium chloride 5 ± 0.1 mM; GDP 1 ± 0.1 mM; TRIS 10 ± 0.1 mM; sodium chloride 10 ± 0.1 mM; DTT 1 ± 0.1 mM; sodium azide 0.01 ± 0.0001 %; roche inhibitors 0.01 ± 0.001 x; EDTA 0.1 ± 0.01 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 293.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.115, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 600 MHz

Related Database Links:

BMRB	10051 17183 17610 17678 18461 18629 25730
PDB	121P 1AA9 1AGP 1BKD 1CLU 1CRP 1CRQ 1CRR 1CTQ 1GNP 1GNQ 1GNR 1HE8 1IAQ 1IOZ 1JAH 1JAI 1K8R 1LF0 1LF5 1LFD 1NVU 1NVV 1NVW 1NVX 1P2S 1P2T 1P2U 1P2V 1PLJ 1PLK 1PLL 1Q21 1QRA 1RVD 1WQ1 1XCM 1XD2 1XJ0 1ZVQ 1ZW6 221P 2C5L 2CE2 2CL0 2CL6 2CL7 2CLC 2CLD 2EVW 2LCF 2LWI 2N42 2N46 2Q21 2QUZ 2RGA 2RGB 2RGC 2RGD 2RGE 2RGG 2UZI 2VH5 2X1V 3DDC 3I3S 3K8Y 3K9L 3K9N 3KKM 3KKN 3KUD 3L8Y 3L8Z 3LBH 3LBI 3LBN 3LO5 3OIU 3OIV 3OIW 3RRY 3RRZ 3RS0 3RS2 3RS3 3RS4 3RS5 3RS7 3RSL 3RSO 3TGP 3V4F 421P 4DLR 4DLS 4DLT 4DLU 4DLV 4DLW 4DLX 4DLY 4DLZ 4EFL 4EFM 4EFN 4G0N 4G3X 4K81 4L9S 4L9W 4NYI 4NYJ 4NYM 4Q21 4RSG 4URU 4URV 4URW 4URX 4URY 4URZ 4US0 4US1 4US2 4XVQ 4XVR 521P 5P21 621P 6Q21 721P 821P
DBJ	BAB61869 BAB61870 BAB88314 BAB88315 BAB88316
EMBL	CAA25322 CAA25624 CAA27258 CAA35240 CAA90306
GB	AAA36554 AAA42009 AAA46568 AAA46569 AAA46570
PIR	A43816 TVMVNS
PRF	0904302A 1604384A
REF	NP_001017003 NP_001018465 NP_001084278 NP_001091711 NP_001123913
SP	P01112 P01113 P01114 P01115 P08642
TPG	DAA13500
AlphaFold	P01112 P01113 P01114 P01115 P08642

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks