BMRB Entry 17183
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17183
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Citation: Iuga, Adriana; Spoerner, Michael; Kalbitzer, Hans; Brunner, Eike. "Solid-state 31P NMR Spectroscopy of Microcrystals of the Ras Protein and its Effector Loop Mutants: Comparison Between Crystalline and Solution State" J. Mol. Biol. 342, 1033-1040 (2004).
PubMed: 15342254
Assembly members:
Ras, polymer, 166 residues, Formula weight is not available
MG, non-polymer, 24.305 Da.
GNP, non-polymer, 522.196 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: ns
- chemical_rates
| Data type | Count |
| kinetic rates | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ras | 1 |
| 2 | MG | 2 |
| 3 | GppNHp | 3 |
Entities:
Entity 1, Ras 166 residues - Formula weight is not available
| 1 | MET | THR | GLU | TYR | LYS | LEU | VAL | VAL | VAL | GLY | ||||
| 2 | ALA | GLY | GLY | VAL | GLY | LYS | SER | ALA | LEU | THR | ||||
| 3 | ILE | GLN | LEU | ILE | GLN | ASN | HIS | PHE | VAL | ASP | ||||
| 4 | GLU | TYR | ASP | PRO | THR | ILE | GLU | ASP | SER | TYR | ||||
| 5 | ARG | LYS | GLN | VAL | VAL | ILE | ASP | GLY | GLU | THR | ||||
| 6 | CYS | LEU | LEU | ASP | ILE | LEU | ASP | THR | ALA | GLY | ||||
| 7 | GLN | GLU | GLU | TYR | SER | ALA | MET | ARG | ASP | GLN | ||||
| 8 | TYR | MET | ARG | THR | GLY | GLU | GLY | PHE | LEU | CYS | ||||
| 9 | VAL | PHE | ALA | ILE | ASN | ASN | THR | LYS | SER | PHE | ||||
| 10 | GLU | ASP | ILE | HIS | GLN | TYR | ARG | GLU | GLN | ILE | ||||
| 11 | LYS | ARG | VAL | LYS | ASP | SER | ASP | ASP | VAL | PRO | ||||
| 12 | MET | VAL | LEU | VAL | GLY | ASN | LYS | CYS | ASP | LEU | ||||
| 13 | ALA | ALA | ARG | THR | VAL | GLU | SER | ARG | GLN | ALA | ||||
| 14 | GLN | ASP | LEU | ALA | ARG | SER | TYR | GLY | ILE | PRO | ||||
| 15 | TYR | ILE | GLU | THR | SER | ALA | LYS | THR | ARG | GLN | ||||
| 16 | GLY | VAL | GLU | ASP | ALA | PHE | TYR | THR | LEU | VAL | ||||
| 17 | ARG | GLU | ILE | ARG | GLN | HIS |
Entity 2, MG - Mg - 24.305 Da.
| 1 | MG |
Entity 3, GppNHp - C10 H17 N6 O13 P3 - 522.196 Da.
| 1 | GNP |
Samples:
sample_1: Ras 1.5 mM; MG 1.5 mM; GppNHp 1.5 mM; Tris-HCl 50 mM; magnesium chloride 10 mM; dithioerythritiol 5 mM; polyethylene glycol 400 56%
sample_conditions_1: pH: 7.6; pressure: ambient atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 31P NMR | sample_1 | anisotropic | sample_conditions_1 |
Software:
SIMPSON, Bak, M., Rasmussen, J. T. & Nielsen, N. C. (2000). - numerical spectra simulations
NMR spectrometers:
- Bruker Avance 300 MHz
Related Database Links:
| BMRB | 10051 17610 17678 18461 18479 18629 25730 |
| PDB | |
| DBJ | BAB61869 BAB61870 BAB88314 BAB88315 BAB88316 |
| EMBL | CAA25322 CAA25624 CAA27258 CAA35240 CAA90306 |
| GB | AAA36554 AAA42009 AAA46568 AAA46569 AAA46570 |
| PIR | A43816 TVMVNS |
| PRF | 0904302A 1604384A |
| REF | NP_001017003 NP_001018465 NP_001084278 NP_001091711 NP_001123913 |
| SP | P01112 P01113 P01114 P01115 P08642 |
| TPG | DAA13500 |
| AlphaFold | P01115 P01113 P01112 P01114 P08642 |