BMRB Entry 10051

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR10051

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Title:
Backbone 1H and 15N assignment for the human c-Ha Ras (Y32W) in the GDP-bound state
Deposition date:
2006-11-13
Original release date:
2007-11-13
Authors:
Matsuda, T.; Koshiba, S.; Tochio, N.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Matsuda, T.; Koshiba, S.; Tochio, N.; Seki, E.; Iwasaki, N.; Yabuki, T.; Inoue, M.; Yokoyama, S.; Kigawa, T.. "Improving cell-free protein synthesis for stable-isotope labeling"  J. Biomol. NMR 37, 225-229 (2007).
PubMed: 17237976

Assembly members:

Assembly members:
RasY32W, polymer, 178 residues, 20034.82 Da.
GDP, non-polymer, 443.201 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RasY32W: GSSGSSGMTEYKLVVVGAGG VGKSALTIQLIQNHFVDEWD PTIEDSYRKQVVIDGETCLL DILDTAGQEEYSAMRDQYMR TGEGFLCVFAINNTKSFEDI HQYREQIKRVKDSDDVPMVL VGNKCDLAARTVESRQAQDL ARSYGIPYIETSAKTRQGVE DAFYTLVREIRQHKLRKL

Data sets:
Data typeCount
15N chemical shifts172
1H chemical shifts172

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RasY32W1
2GDP2

Entities:

Entity 1, RasY32W 178 residues - 20034.82 Da.

1   GLYSERSERGLYSERSERGLYMETTHRGLU
2   TYRLYSLEUVALVALVALGLYALAGLYGLY
3   VALGLYLYSSERALALEUTHRILEGLNLEU
4   ILEGLNASNHISPHEVALASPGLUTRPASP
5   PROTHRILEGLUASPSERTYRARGLYSGLN
6   VALVALILEASPGLYGLUTHRCYSLEULEU
7   ASPILELEUASPTHRALAGLYGLNGLUGLU
8   TYRSERALAMETARGASPGLNTYRMETARG
9   THRGLYGLUGLYPHELEUCYSVALPHEALA
10   ILEASNASNTHRLYSSERPHEGLUASPILE
11   HISGLNTYRARGGLUGLNILELYSARGVAL
12   LYSASPSERASPASPVALPROMETVALLEU
13   VALGLYASNLYSCYSASPLEUALAALAARG
14   THRVALGLUSERARGGLNALAGLNASPLEU
15   ALAARGSERTYRGLYILEPROTYRILEGLU
16   THRSERALALYSTHRARGGLNGLYVALGLU
17   ASPALAPHETYRTHRLEUVALARGGLUILE
18   ARGGLNHISLYSLEUARGLYSLEU

Entity 2, GDP - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Samples:

sample_1: RasY32W, [U-13C; U-15N], 1 mM; sodium phosphate 20 mM; NaCl 100 mM; d-DTT 5 mM; magnesium chloride 5 mM; D2O 10%; H2O 90%; sodium azide 0.01%

condition_1: ionic strength: 0.12 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H15N-HSQCsample_1not availablecondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.913, Kobayashi, N. - data analysis

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

BMRB 17183 17610 17678 18461 18479 18629 25730
PDB
DBJ BAB61869 BAB61870 BAB88314 BAB88315 BAB88316
EMBL CAA25322 CAA25624 CAA27258 CAA90306 CAD24594
GB AAA42009 AAA46568 AAA46569 AAA46570 AAA46574
PIR A43816 TVMVNS
PRF 0904302A 1604384A
REF NP_001017003 NP_001018465 NP_001084278 NP_001091711 NP_001123913
SP P01112 P01113 P01114 P01115 P08642
TPG DAA13500
AlphaFold P01112 P01113 P01114 P01115 P08642

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks