BMRB Entry 17610
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PDB ID: 2n46
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17610
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Araki, Mitsugu; Shima, Fumi; Yoshikawa, Yoko; Muraoka, Shin; Ijiri, Yuichi; Nagahara, Yuka; Shirono, Tomoya; Kataoka, Tohru; Tamura, Atsuo. "Solution structure of the state 1 conformer of GTP-bound H-Ras protein and distinct dynamic properties between the state 1 and state 2 conformers." J. Biol. Chem. 286, 39644-39653 (2011).
PubMed: 21930707
Assembly members:
GppNHp-bound H-RasT35S mutant protein, polymer, 172 residues, 18861.303 Da.
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER, non-polymer, 522.196 Da.
MG, non-polymer, 24.305 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P
Entity Sequences (FASTA):
GppNHp-bound H-RasT35S mutant protein: GPLGSDMTEYKLVVVGAGGV
GKSALTIQLIQNHFVDEYDP
SIEDSYRKQVVIDGETCLLD
ILDTAGQEEYSAMRDQYMRT
GEGFLCVFAINNTKSFEDIH
QYREQIKRVKDSDDVPMVLV
GNKCDLAARTVESRQAQDLA
RSYGIPYIETSAKTRQGVED
AFYTLVREIRQH
- assigned_chemical_shifts
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| 13C chemical shifts | 719 |
| 15N chemical shifts | 184 |
| 1H chemical shifts | 1177 |
| T1 relaxation values | 130 |
| T2 relaxation values | 130 |
| heteronuclear NOE values | 130 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GppNHp-bound H-RasT35S mutant protein | 1 |
| 2 | PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | 2 |
| 3 | magnesium ions | 3 |
Entities:
Entity 1, GppNHp-bound H-RasT35S mutant protein 172 residues - 18861.303 Da.
| 1 | GLY | PRO | LEU | GLY | SER | ASP | MET | THR | GLU | TYR | ||||
| 2 | LYS | LEU | VAL | VAL | VAL | GLY | ALA | GLY | GLY | VAL | ||||
| 3 | GLY | LYS | SER | ALA | LEU | THR | ILE | GLN | LEU | ILE | ||||
| 4 | GLN | ASN | HIS | PHE | VAL | ASP | GLU | TYR | ASP | PRO | ||||
| 5 | SER | ILE | GLU | ASP | SER | TYR | ARG | LYS | GLN | VAL | ||||
| 6 | VAL | ILE | ASP | GLY | GLU | THR | CYS | LEU | LEU | ASP | ||||
| 7 | ILE | LEU | ASP | THR | ALA | GLY | GLN | GLU | GLU | TYR | ||||
| 8 | SER | ALA | MET | ARG | ASP | GLN | TYR | MET | ARG | THR | ||||
| 9 | GLY | GLU | GLY | PHE | LEU | CYS | VAL | PHE | ALA | ILE | ||||
| 10 | ASN | ASN | THR | LYS | SER | PHE | GLU | ASP | ILE | HIS | ||||
| 11 | GLN | TYR | ARG | GLU | GLN | ILE | LYS | ARG | VAL | LYS | ||||
| 12 | ASP | SER | ASP | ASP | VAL | PRO | MET | VAL | LEU | VAL | ||||
| 13 | GLY | ASN | LYS | CYS | ASP | LEU | ALA | ALA | ARG | THR | ||||
| 14 | VAL | GLU | SER | ARG | GLN | ALA | GLN | ASP | LEU | ALA | ||||
| 15 | ARG | SER | TYR | GLY | ILE | PRO | TYR | ILE | GLU | THR | ||||
| 16 | SER | ALA | LYS | THR | ARG | GLN | GLY | VAL | GLU | ASP | ||||
| 17 | ALA | PHE | TYR | THR | LEU | VAL | ARG | GLU | ILE | ARG | ||||
| 18 | GLN | HIS |
Entity 2, PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER - C10 H17 N6 O13 P3 - 522.196 Da.
| 1 | GNP |
Entity 3, magnesium ions - Mg - 24.305 Da.
| 1 | MG |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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