BMRB Entry 17678

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17678

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Title:
HRas166*GppNHp backbone chemical shift assignments
Deposition date:
2011-05-31
Original release date:
2011-09-19
Authors:
Kovrigin, Evgenii; O'Connor, Casey
Citation:

Citation: Kovrigin, Casey. "Assignments of backbone (1)H, (13)C and (15)N resonances in H-Ras (1-166) complexed with GppNHp at physiological pH."  Biomol. NMR Assignments 6, 91-93 (2012).
PubMed: 21814767

Assembly members:

Assembly members:
HRas166, polymer, 166 residues, 18853.2 Da.
GNP, non-polymer, 522.196 Da.
MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HRas166: MTEYKLVVVGAGGVGKSALT IQLIQNHFVDEYDPTIEDSY RKQVVIDGETCLLDILDTAG QEEYSAMRDQYMRTGEGFLC VFAINNTKSFEDIHQYREQI KRVKDSDDVPMVLVGNKCDL AARTVESRQAQDLARSYGIP YIETSAKTRQGVEDAFYTLV REIRQH

Data sets:
Data typeCount
13C chemical shifts389
15N chemical shifts128
1H chemical shifts127

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1G-domain1
2GNP2
3MG3

Entities:

Entity 1, G-domain 166 residues - 18853.2 Da.

1   METTHRGLUTYRLYSLEUVALVALVALGLY
2   ALAGLYGLYVALGLYLYSSERALALEUTHR
3   ILEGLNLEUILEGLNASNHISPHEVALASP
4   GLUTYRASPPROTHRILEGLUASPSERTYR
5   ARGLYSGLNVALVALILEASPGLYGLUTHR
6   CYSLEULEUASPILELEUASPTHRALAGLY
7   GLNGLUGLUTYRSERALAMETARGASPGLN
8   TYRMETARGTHRGLYGLUGLYPHELEUCYS
9   VALPHEALAILEASNASNTHRLYSSERPHE
10   GLUASPILEHISGLNTYRARGGLUGLNILE
11   LYSARGVALLYSASPSERASPASPVALPRO
12   METVALLEUVALGLYASNLYSCYSASPLEU
13   ALAALAARGTHRVALGLUSERARGGLNALA
14   GLNASPLEUALAARGSERTYRGLYILEPRO
15   TYRILEGLUTHRSERALALYSTHRARGGLN
16   GLYVALGLUASPALAPHETYRTHRLEUVAL
17   ARGGLUILEARGGLNHIS

Entity 2, GNP - C10 H17 N6 O13 P3 - 522.196 Da.

1   GNP

Entity 3, MG - Mg - 24.305 Da.

1   MG

Samples:

sample_1: HRas166, [U-99% 13C; U-99% 15N], 1.05 ± 0.1 mM; magnesium chloride 5 ± 0.1 mM; GNP 1 ± 0.1 mM; TRIS 10 ± 0.1 mM; sodium chloride 10 ± 0.1 mM; DTT 1 ± 0.1 mM; sodium azide 0.01 ± 0.0001 %; roche inhibitors 0.1 ± 0.001 x; EDTA 0.1 ± 0.01 mM; H20 90%; D20 10%

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 293.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY v3.115, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 10051 17183 17610 18461 18479 18629 25730
PDB
DBJ BAB61869 BAB61870 BAB88314 BAB88315 BAB88316
EMBL CAA25322 CAA25624 CAA27258 CAA35240 CAA90306
GB AAA36554 AAA42009 AAA46568 AAA46569 AAA46570
PIR A43816 TVMVNS
PRF 0904302A 1604384A
REF NP_001017003 NP_001018465 NP_001084278 NP_001091711 NP_001123913
SP P01112 P01113 P01114 P01115 P08642
TPG DAA13500
AlphaFold P01112 P01113 P01114 P01115 P08642

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks